Detailed information of TA system TA151000

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3369977..3370812
• Replicon: chromosome
• Accession: NZ_CP049201
• Organism: Escherichia coli strain PapRG-04-4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: G6Z99_RS16400
• Protein ID: WP_164475869.1
• Coordinates: 3369977..3370354 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: G6Z99_RS16405
• Protein ID: WP_164475870.1
• Coordinates: 3370444..3370812 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z99_RS16370	3366646..3367350	+	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-
G6Z99_RS16375	3367635..3367853	+	219	WP_001040187.1	translation initiation factor IF-1	-
G6Z99_RS16385	3368344..3369186	-	843	WP_164475867.1	DUF4942 domain-containing protein	-
G6Z99_RS16390	3369283..3369480	-	198	WP_000839263.1	DUF957 domain-containing protein	-
G6Z99_RS16395	3369492..3369980	-	489	WP_164475868.1	hypothetical protein	-
G6Z99_RS16400	3369977..3370354	-	378	WP_164475869.1	TA system toxin CbtA family protein	Toxin
G6Z99_RS16405	3370444..3370812	-	369	WP_164475870.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G6Z99_RS16410	3370887..3371108	-	222	WP_000692300.1	DUF987 domain-containing protein	-
G6Z99_RS16415	3371177..3371653	-	477	WP_001424026.1	RadC family protein	-
G6Z99_RS16420	3371669..3372154	-	486	WP_001563926.1	antirestriction protein	-
G6Z99_RS16425	3372246..3373064	-	819	WP_164475828.1	DUF945 domain-containing protein	-
G6Z99_RS16430	3373339..3373794	-	456	WP_097754558.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.05 Da
• Isoelectric Point: 7.3842

>T151000 WP_164475869.1 NZ_CP049201:c3370354-3369977 [Escherichia coli]
MKTLPDTHVREASRSPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T151000 NZ_CP065172:1929580-1929682 [Klebsiella pneumoniae]
GCAAGGCGGCTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13687.55 Da
• Isoelectric Point: 7.3715

>AT151000 WP_164475870.1 NZ_CP049201:c3370812-3370444 [Escherichia coli]
VSDTLHETTHPDDNRGRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT151000 NZ_CP065172:c1929689-1929544 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGCCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT