Detailed information of TA system TA150999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3126101..3127821
• Replicon: chromosome
• Accession: NZ_CP049201
• Organism: Escherichia coli strain PapRG-04-4

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A2T3TQG6
• Locus tag: G6Z99_RS15355
• Protein ID: WP_024224217.1
• Coordinates: 3126101..3126757 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: B7UP19
• Locus tag: G6Z99_RS15360
• Protein ID: WP_000634203.1
• Coordinates: 3126754..3127821 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z99_RS15335	3121495..3121749	-	255	WP_154670580.1	hypothetical protein	-
G6Z99_RS15340	3122031..3122270	+	240	WP_001431807.1	hypothetical protein	-
G6Z99_RS15345	3122310..3124748	+	2439	WP_053264748.1	type I restriction-modification system subunit M	-
G6Z99_RS15350	3124748..3126088	+	1341	WP_053264747.1	restriction endonuclease subunit S	-
G6Z99_RS15355	3126101..3126757	+	657	WP_024224217.1	DUF4433 domain-containing protein	Toxin
G6Z99_RS15360	3126754..3127821	+	1068	WP_000634203.1	macro domain-containing protein	Antitoxin
G6Z99_RS15365	3127840..3130935	+	3096	WP_053264746.1	HsdR family type I site-specific deoxyribonuclease	-
G6Z99_RS15370	3130935..3131651	+	717	WP_001122107.1	M48 family metallopeptidase	-
G6Z99_RS15375	3132259..3132816	-	558	WP_000736772.1	Ail/Lom family outer membrane beta-barrel protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3120088..3141485	21397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25232.57 Da
• Isoelectric Point: 6.4006

>T150999 WP_024224217.1 NZ_CP049201:3126101-3126757 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLNSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Nucleotide

• Length: 657 bp

>T150999 NZ_CP065172:783130-783540 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40672.70 Da
• Isoelectric Point: 5.6927

>AT150999 WP_000634203.1 NZ_CP049201:3126754-3127821 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT150999 NZ_CP065172:782883-783149 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A2T3TQG6

Antitoxin

• AlphaFold DB: A0A7U9J2B9