Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 993720..994374 Replicon chromosome
Accession NZ_CP049201
Organism Escherichia coli strain PapRG-04-4

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag G6Z99_RS04750 Protein ID WP_000244781.1
Coordinates 993967..994374 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G6Z99_RS04745 Protein ID WP_000354046.1
Coordinates 993720..993986 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Z99_RS04725 989808..991241 - 1434 WP_021557183.1 6-phospho-beta-glucosidase BglA -
G6Z99_RS04730 991286..991597 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
G6Z99_RS04735 991761..992420 + 660 WP_000250274.1 hemolysin III family protein -
G6Z99_RS04740 992497..993477 - 981 WP_000886074.1 tRNA-modifying protein YgfZ -
G6Z99_RS04745 993720..993986 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G6Z99_RS04750 993967..994374 + 408 WP_000244781.1 protein YgfX Toxin
G6Z99_RS04755 994414..994935 - 522 WP_075335445.1 flavodoxin FldB -
G6Z99_RS04760 995047..995943 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G6Z99_RS04765 995967..996677 + 711 WP_106905212.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G6Z99_RS04770 996683..998416 + 1734 WP_164475476.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T150985 WP_000244781.1 NZ_CP049201:993967-994374 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T150985 NZ_CP065167:364225-364572 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT150985 WP_000354046.1 NZ_CP049201:993720-993986 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT150985 NZ_CP065167:364572-364871 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References