Detailed information of TA system TA150983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 776551..777886
• Replicon: chromosome
• Accession: NZ_CP049201
• Organism: Escherichia coli strain PapRG-04-4

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: G6Z99_RS03740
• Protein ID: WP_057697651.1
• Coordinates: 776551..777498 (-)
• Length: 316 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A0Q1C5A3
• Locus tag: G6Z99_RS03745
• Protein ID: WP_000208385.1
• Coordinates: 777491..777886 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z99_RS03710	771976..772539	+	564	WP_164475438.1	type II secretion system minor pseudopilin GspH	-
G6Z99_RS03715	772536..772907	+	372	WP_001718860.1	type II secretion system minor pseudopilin GspI	-
G6Z99_RS03720	772904..773509	+	606	WP_069913163.1	type II secretion system minor pseudopilin GspJ	-
G6Z99_RS03725	773506..774483	+	978	WP_069913164.1	type II secretion system minor pseudopilin GspK	-
G6Z99_RS03730	774480..775658	+	1179	WP_069913165.1	type II secretion system protein GspL	-
G6Z99_RS03735	775660..776196	+	537	WP_000942790.1	GspM family type II secretion system protein YghD	-
G6Z99_RS03740	776551..777498	-	948	WP_057697651.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
G6Z99_RS03745	777491..777886	-	396	WP_000208385.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
G6Z99_RS03750	777955..778800	-	846	WP_158179441.1	DUF4942 domain-containing protein	-
G6Z99_RS03755	778885..779082	-	198	WP_000839249.1	DUF957 domain-containing protein	-
G6Z99_RS03760	779094..779582	-	489	WP_164475439.1	hypothetical protein	-
G6Z99_RS03765	779579..779956	-	378	WP_057698567.1	TA system toxin CbtA family protein	-
G6Z99_RS03770	780003..780377	-	375	WP_057698646.1	type IV toxin-antitoxin system YeeU family antitoxin	-
G6Z99_RS03775	780453..780674	-	222	WP_000692300.1	DUF987 domain-containing protein	-
G6Z99_RS03780	780743..781219	-	477	WP_001424026.1	RadC family protein	-
G6Z99_RS03785	781235..781720	-	486	WP_001563926.1	antirestriction protein	-
G6Z99_RS03790	781812..782630	-	819	WP_057697860.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	rfaE / gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / papC / papD / vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH	671286..908004	236718

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-302)

Antitoxin

• Protein of unknown function DUF6088 (10-129)

Sequences

Toxin

Protein

• Length: 316 a.a.
• Molecular weight: 36240.66 Da
• Isoelectric Point: 6.3550

>T150983 WP_057697651.1 NZ_CP049201:c777498-776551 [Escherichia coli]
MPELTSLFPDVANALDIESVAIVEKDYFVVNLLKLLKEIKPETHTLVFAGGTALSKAGISLNRMSEDIDIKLVPTENFMQ
NGRDKRRKIRKKIVQIITDVINNSDIFSLDNENARITRDEYRYNEISVRYPQTFAQVPCLRPFIKLELMESTLLEHPESR
DIYSLVTELTGKGTPVTAFPCATILSTQAEKLISMMRRTAAHLRNPEQQDDEFLVRHIYDNYCIVREKGVNVPVLKNFVQ
ICIQQDIERYGNQYPQFCSSPVDELKKGLDALRDDPVHKLRFQRFVKPMVFGNTQIDWEEAYSCFRQTALSVLTS

Nucleotide

• Length: 948 bp

>T150983 NZ_CP065164:53790-54272 [Klebsiella variicola]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14595.97 Da
• Isoelectric Point: 10.9440

>AT150983 WP_000208385.1 NZ_CP049201:c777886-777491 [Escherichia coli]
MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEGKLMKVGYGVYTKARRNVITGKIMPAAPGGSAAVITE
ALDLLNIRYSLIGATECYNKGQSTQIPVSPEFKTPPRFKRVLVVGKSKINA

Nucleotide

• Length: 396 bp

>AT150983 NZ_CP065164:53536-53802 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0Q1C5A3