Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 5025738..5026392 Replicon chromosome
Accession NZ_CP049198
Organism Escherichia coli strain E686

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag G6000_RS23875 Protein ID WP_000244781.1
Coordinates 5025985..5026392 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G6000_RS23870 Protein ID WP_000354046.1
Coordinates 5025738..5026004 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6000_RS23845 5020899..5021641 + 743 Protein_4647 SDR family oxidoreductase -
G6000_RS23850 5021698..5023131 - 1434 WP_001605840.1 6-phospho-beta-glucosidase BglA -
G6000_RS23855 5023176..5023487 + 312 WP_001182943.1 N(4)-acetylcytidine aminohydrolase -
G6000_RS23860 5023651..5024310 + 660 WP_000250283.1 hemolysin III family protein -
G6000_RS23865 5024506..5025486 - 981 WP_000886102.1 tRNA-modifying protein YgfZ -
G6000_RS23870 5025738..5026004 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G6000_RS23875 5025985..5026392 + 408 WP_000244781.1 protein YgfX Toxin
G6000_RS23880 5026432..5026953 - 522 WP_001055874.1 flavodoxin FldB -
G6000_RS23885 5027065..5027961 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G6000_RS23890 5027986..5028696 + 711 WP_000715223.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G6000_RS23895 5028702..5030435 + 1734 WP_000813232.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-131)

Antitoxin

(7-79)


Sequences


Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T150981 WP_000244781.1 NZ_CP049198:5025985-5026392 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T150981 NZ_CP065162:c5495548-5495165 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT150981 WP_000354046.1 NZ_CP049198:5025738-5026004 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT150981 NZ_CP065162:c5495790-5495548 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1PAM6


Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References