Detailed information of TA system TA150972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3138205..3138692
• Replicon: chromosome
• Accession: NZ_CP049198
• Organism: Escherichia coli strain E686

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: G6000_RS14900
• Protein ID: WP_001520461.1
• Coordinates: 3138205..3138456 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2T1MJK4
• Locus tag: G6000_RS14905
• Protein ID: WP_001520458.1
• Coordinates: 3138459..3138692 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6000_RS14875	3133817..3135103	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
G6000_RS14880	3135103..3136092	+	990	WP_001520462.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
G6000_RS14885	3136089..3136910	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
G6000_RS14890	3136913..3137290	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
G6000_RS14895	3137297..3138145	+	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
G6000_RS14900	3138205..3138456	-	252	WP_001520461.1	CcdB family protein	Toxin
G6000_RS14905	3138459..3138692	-	234	WP_001520458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
G6000_RS14910	3138778..3139257	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
G6000_RS14915	3139449..3141311	-	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
G6000_RS14920	3141304..3141834	-	531	WP_021522993.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
G6000_RS14925	3141942..3143273	-	1332	WP_001520455.1	MFS transporter	-
G6000_RS14930	3143332..3143619	-	288	WP_000203747.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9482.12 Da
• Isoelectric Point: 10.4134

>T150972 WP_001520461.1 NZ_CP049198:c3138456-3138205 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T150972 NZ_CP065162:346612-346959 [Klebsiella variicola]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGGTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.61 Da
• Isoelectric Point: 5.8062

>AT150972 WP_001520458.1 NZ_CP049198:c3138692-3138459 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT150972 NZ_CP065162:346959-347258 [Klebsiella variicola]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGATCCGCGGCTTTCGACCCTAAAACGCTACATTGCGGCACTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2T1MJK4