Detailed information of TA system TA150966

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1980039..1980883
• Replicon: chromosome
• Accession: NZ_CP049198
• Organism: Escherichia coli strain E686

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: G6000_RS09685
• Protein ID: WP_000854686.1
• Coordinates: 1980039..1980422 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: G6000_RS09690
• Protein ID: WP_001285602.1
• Coordinates: 1980503..1980883 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6000_RS09645	1975043..1975534	-	492	WP_001308552.1	DUF1097 domain-containing protein	-
G6000_RS09650	1975636..1976190	-	555	WP_001001921.1	molecular chaperone YcdY	-
G6000_RS09655	1976214..1976951	-	738	WP_000283664.1	zinc-binding phosphatase	-
G6000_RS09660	1977006..1977944	-	939	WP_001522839.1	glyoxylate/hydroxypyruvate reductase GhrA	-
G6000_RS09670	1978414..1979255	-	842	Protein_1898	DUF4942 domain-containing protein	-
G6000_RS09675	1979340..1979537	-	198	WP_000839253.1	DUF957 domain-containing protein	-
G6000_RS09680	1979554..1980042	-	489	WP_032180032.1	hypothetical protein	-
G6000_RS09685	1980039..1980422	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
G6000_RS09690	1980503..1980883	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G6000_RS09695	1980894..1981577	-	684	WP_000086768.1	hypothetical protein	-
G6000_RS09700	1981596..1981817	-	222	WP_000692298.1	DUF987 domain-containing protein	-
G6000_RS09705	1981880..1982356	-	477	WP_001186726.1	RadC family protein	-
G6000_RS09710	1982372..1982857	-	486	WP_000214307.1	antirestriction protein	-
G6000_RS09715	1982949..1983770	-	822	WP_001761104.1	DUF945 domain-containing protein	-
G6000_RS09720	1983871..1984079	-	209	Protein_1908	DUF905 family protein	-
G6000_RS09725	1984180..1984635	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	blaCMY-2	csgB / csgD / csgE / csgF / csgG	1971435..2027389	55954

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T150966 WP_000854686.1 NZ_CP049198:c1980422-1980039 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T150966 NZ_CP065154:1399794-1400096 [Lacticaseibacillus paracasei subsp. tolerans]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCCGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCCT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTATATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTGAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACCGGATCTCATCATAACCTTCTGGTTAAATAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT150966 WP_001285602.1 NZ_CP049198:c1980883-1980503 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT150966 NZ_CP065154:1399532-1399801 [Lacticaseibacillus paracasei subsp. tolerans]
ATGGCAGCCACAAAGAAGGAAACTCGCTTGAATATTCGTGTTGATCCTGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG