Detailed information of TA system TA150965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1544824..1545462
• Replicon: chromosome
• Accession: NZ_CP049198
• Organism: Escherichia coli strain E686

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: G6000_RS07465
• Protein ID: WP_001306887.1
• Coordinates: 1545286..1545462 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: G6000_RS07460
• Protein ID: WP_001270286.1
• Coordinates: 1544824..1545240 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6000_RS07440	1539977..1540918	-	942	WP_001251315.1	ABC transporter permease	-
G6000_RS07445	1540919..1541932	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
G6000_RS07450	1541910..1543094	-	1185	WP_164477118.1	ABC transporter substrate-binding protein	-
G6000_RS07455	1543339..1544745	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
G6000_RS07460	1544824..1545240	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
G6000_RS07465	1545286..1545462	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
G6000_RS07470	1545684..1545914	+	231	WP_000494241.1	YncJ family protein	-
G6000_RS07475	1546006..1547967	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
G6000_RS07480	1548040..1548576	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
G6000_RS07485	1548668..1549839	+	1172	Protein_1469	BenE family transporter YdcO	-
G6000_RS07490	1549947..1550405	-	459	WP_000526135.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1549947..1550405	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T150965 WP_001306887.1 NZ_CP049198:c1545462-1545286 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T150965 NZ_CP065154:668368-668649 [Lacticaseibacillus paracasei subsp. tolerans]
ATGAATAAATTTGTTTATAAACCAGCCTTTGAACGTCAATTTAAAAAACATTACAAGACGATTTTAAAAGGGGGGCGCTA
TAAAAAAGAAGATTTTGAAGCTGTTTATCATAAACTATTACGAGATGAACCGTTAGATCCGCATTATAATGATCACGCTT
TGGTTAATCGTAAACCAGAGCGTGATTTACATATCAAGCCGGATTGGTTATTAATTTACAAATATGATGGTGAGTTTGTG
CGCTTTATCGACACGGGTTCGCATTCAGATCTTTTTAACTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT150965 WP_001270286.1 NZ_CP049198:c1545240-1544824 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT150965 NZ_CP065154:668133-668375 [Lacticaseibacillus paracasei subsp. tolerans]
ATGACTAGCCAAGTACATTTCAGAATGAGCGAAAAAGAAAAAAAGGACTTCGAGATTGTCCTAAGTCGTGTGGGGTTAAC
TCCCGGGGAAGCTTTTCGTATCTTTGCCAAGAAATCCATTGAAGCCGGAGGAATTCCCTTTGAGGTCTCTCAGCCAAACA
CACGTTTAAAAAAGGCTATTAGGAGTCAAGATTACGTTGAATTTAACGATCCTGAGGAAGGACTGGATTGGTTAAATGAA
TAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76