Detailed information of TA system TA150964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1363705..1364231
• Replicon: chromosome
• Accession: NZ_CP049198
• Organism: Escherichia coli strain E686

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: G6000_RS06570
• Protein ID: WP_000323025.1
• Coordinates: 1363944..1364231 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: G6000_RS06565
• Protein ID: WP_000534858.1
• Coordinates: 1363705..1363944 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6000_RS06540	1358967..1359581	+	615	WP_001265627.1	hypothetical protein	-
G6000_RS06545	1359578..1360606	+	1029	WP_001345551.1	DUF4868 domain-containing protein	-
G6000_RS06550	1361090..1362403	+	1314	WP_000589005.1	ISNCY family transposase	-
G6000_RS06555	1362840..1363172	-	333	WP_001301033.1	protein FlxA	-
G6000_RS06560	1363375..1363680	-	306	WP_001326990.1	hypothetical protein	-
G6000_RS06565	1363705..1363944	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
G6000_RS06570	1363944..1364231	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
G6000_RS06575	1364303..1364458	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
G6000_RS06580	1364675..1364926	+	252	WP_000980994.1	hypothetical protein	-
G6000_RS06585	1364993..1365271	+	279	WP_012304870.1	hypothetical protein	-
G6000_RS06590	1365273..1366322	+	1050	WP_001265199.1	DUF968 domain-containing protein	-
G6000_RS06595	1366336..1367088	+	753	WP_001047135.1	antitermination protein	-
G6000_RS06600	1367366..1367455	-	90	WP_120795389.1	hypothetical protein	-
G6000_RS06605	1367510..1367722	-	213	WP_000087756.1	cold shock-like protein CspF	-
G6000_RS06610	1368023..1368238	+	216	WP_000066484.1	cold shock-like protein CspB	-
G6000_RS06615	1368992..1369207	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1337562..1399834	62272

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T150964 WP_000323025.1 NZ_CP049198:1363944-1364231 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T150964 NZ_CP065153:189088-189504 [Escherichia coli O150:H6]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT150964 WP_000534858.1 NZ_CP049198:1363705-1363944 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT150964 NZ_CP065153:188861-189091 [Escherichia coli O150:H6]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8