Detailed information of TA system TA150949

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3867938..3868773
• Replicon: chromosome
• Accession: NZ_CP049197
• Organism: Escherichia coli strain E597

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9IW37
• Locus tag: G6Z98_RS18425
• Protein ID: WP_023356554.1
• Coordinates: 3868396..3868773 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: G6Z98_RS18420
• Protein ID: WP_001295723.1
• Coordinates: 3867938..3868306 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z98_RS18385	3863602..3864282	+	681	WP_001282921.1	WYL domain-containing protein	-
G6Z98_RS18390	3864430..3865107	+	678	WP_001097302.1	hypothetical protein	-
G6Z98_RS18395	3865113..3865346	+	234	WP_001278293.1	DUF905 family protein	-
G6Z98_RS18400	3865436..3866254	+	819	WP_001175165.1	DUF945 domain-containing protein	-
G6Z98_RS18405	3866520..3866999	+	480	WP_000706978.1	antirestriction protein	-
G6Z98_RS18410	3867015..3867491	+	477	WP_001186774.1	RadC family protein	-
G6Z98_RS18415	3867554..3867775	+	222	WP_023356553.1	DUF987 domain-containing protein	-
G6Z98_RS18420	3867938..3868306	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G6Z98_RS18425	3868396..3868773	+	378	WP_023356554.1	TA system toxin CbtA family protein	Toxin
G6Z98_RS18430	3868770..3868919	+	150	Protein_3602	hypothetical protein	-
G6Z98_RS18435	3868998..3869192	+	195	WP_024181941.1	DUF957 domain-containing protein	-
G6Z98_RS18440	3869277..3870119	+	843	WP_022645115.1	DUF4942 domain-containing protein	-
G6Z98_RS18445	3870868..3872406	+	1539	WP_001187196.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3834431..3880218	45787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14052.01 Da
• Isoelectric Point: 7.8045

>T150949 WP_023356554.1 NZ_CP049197:3868396-3868773 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFADERVIEQHIEAGIALCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T150949 NZ_CP065152:c2697755-2697537 [Escherichia coli O150:H6]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT150949 WP_001295723.1 NZ_CP049197:3867938-3868306 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT150949 NZ_CP065152:c2698155-2697781 [Escherichia coli O150:H6]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7U9IW37

Antitoxin

• AlphaFold DB: S1NM52