Detailed information of TA system TA150945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3435950..3436437
• Replicon: chromosome
• Accession: NZ_CP049197
• Organism: Escherichia coli strain E597

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: G6Z98_RS16250
• Protein ID: WP_001520461.1
• Coordinates: 3435950..3436201 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2T1MJK4
• Locus tag: G6Z98_RS16255
• Protein ID: WP_001520458.1
• Coordinates: 3436204..3436437 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z98_RS16225	3431562..3432848	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
G6Z98_RS16230	3432848..3433837	+	990	WP_001520462.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
G6Z98_RS16235	3433834..3434655	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
G6Z98_RS16240	3434658..3435035	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
G6Z98_RS16245	3435042..3435890	+	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
G6Z98_RS16250	3435950..3436201	-	252	WP_001520461.1	CcdB family protein	Toxin
G6Z98_RS16255	3436204..3436437	-	234	WP_001520458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
G6Z98_RS16260	3436523..3437002	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
G6Z98_RS16265	3437194..3439056	-	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
G6Z98_RS16270	3439049..3439579	-	531	WP_021522993.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
G6Z98_RS16275	3439687..3441018	-	1332	WP_001520455.1	MFS transporter	-
G6Z98_RS16280	3441077..3441364	-	288	WP_000203747.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9482.12 Da
• Isoelectric Point: 10.4134

>T150945 WP_001520461.1 NZ_CP049197:c3436201-3435950 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T150945 NZ_CP065152:c1875818-1875441 [Escherichia coli O150:H6]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.61 Da
• Isoelectric Point: 5.8062

>AT150945 WP_001520458.1 NZ_CP049197:c3436437-3436204 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT150945 NZ_CP065152:c1876239-1875865 [Escherichia coli O150:H6]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2T1MJK4