Detailed information of TA system TA150940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2298286..2300006
• Replicon: chromosome
• Accession: NZ_CP049197
• Organism: Escherichia coli strain E597

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A7U9J2A7
• Locus tag: G6Z98_RS11135
• Protein ID: WP_000312833.1
• Coordinates: 2298286..2298942 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: B7UP19
• Locus tag: G6Z98_RS11140
• Protein ID: WP_000634203.1
• Coordinates: 2298939..2300006 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z98_RS11120	2294201..2294440	+	240	WP_024224214.1	hypothetical protein	-
G6Z98_RS11125	2294480..2296918	+	2439	WP_032180025.1	type I restriction-modification system subunit M	-
G6Z98_RS11130	2296930..2298273	+	1344	WP_032180130.1	restriction endonuclease subunit S	-
G6Z98_RS11135	2298286..2298942	+	657	WP_000312833.1	DUF4433 domain-containing protein	Toxin
G6Z98_RS11140	2298939..2300006	+	1068	WP_000634203.1	macro domain-containing protein	Antitoxin
G6Z98_RS11145	2300025..2303120	+	3096	WP_000108736.1	HsdR family type I site-specific deoxyribonuclease	-
G6Z98_RS11150	2303120..2303836	+	717	WP_001122107.1	M48 family metallopeptidase	-
G6Z98_RS11155	2304181..2304795	+	615	Protein_2186	IS3-like element IS600 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	blaCMY-2	-	2290848..2326432	35584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25233.56 Da
• Isoelectric Point: 6.2145

>T150940 WP_000312833.1 NZ_CP049197:2298286-2298942 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Nucleotide

• Length: 657 bp

>T150940 NZ_CP065152:499824-500201 [Escherichia coli O150:H6]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCAGCCCATCTCCAGTAACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTAAATGACACACCGTTCTCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCCCGGTTC
AGCACTAGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCTAAACGATGA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40672.70 Da
• Isoelectric Point: 5.6927

>AT150940 WP_000634203.1 NZ_CP049197:2298939-2300006 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT150940 NZ_CP065152:499366-499734 [Escherichia coli O150:H6]
GTGTCAGACACGCACCATGAGACAAATTATCCCGACGACTATAACAACCGCCCCTGGTGGGGGCTGCCCTGCACCATAAC
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGACTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9J2A7

Antitoxin

• AlphaFold DB: A0A7U9J2B9