Detailed information of TA system TA150938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2232340..2233124
• Replicon: chromosome
• Accession: NZ_CP049197
• Organism: Escherichia coli strain E597

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: G6Z98_RS10765
• Protein ID: WP_000613626.1
• Coordinates: 2232630..2233124 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: G6Z98_RS10760
• Protein ID: WP_001110447.1
• Coordinates: 2232340..2232633 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z98_RS10750	2227490..2228449	-	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
G6Z98_RS10755	2229022..2232207	+	3186	WP_001522867.1	ribonuclease E	-
G6Z98_RS10760	2232340..2232633	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
G6Z98_RS10765	2232630..2233124	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
G6Z98_RS10770	2233219..2234172	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
G6Z98_RS10775	2234184..2235827	-	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
G6Z98_RS10780	2235893..2236834	-	942	WP_001522863.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
G6Z98_RS10785	2236834..2237931	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T150938 WP_000613626.1 NZ_CP049197:2232630-2233124 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T150938 NZ_CP065152:c187750-187415 [Escherichia coli O150:H6]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT150938 WP_001110447.1 NZ_CP049197:2232340-2232633 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT150938 NZ_CP065152:c187998-187750 [Escherichia coli O150:H6]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6