Detailed information of TA system TA150937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1840257..1840895
• Replicon: chromosome
• Accession: NZ_CP049197
• Organism: Escherichia coli strain E597

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: G6Z98_RS08805
• Protein ID: WP_001306887.1
• Coordinates: 1840719..1840895 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: G6Z98_RS08800
• Protein ID: WP_001270286.1
• Coordinates: 1840257..1840673 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z98_RS08780	1835409..1836350	-	942	WP_001251315.1	ABC transporter permease	-
G6Z98_RS08785	1836351..1837364	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
G6Z98_RS08790	1837382..1838527	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
G6Z98_RS08795	1838772..1840178	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
G6Z98_RS08800	1840257..1840673	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
G6Z98_RS08805	1840719..1840895	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
G6Z98_RS08810	1841117..1841347	+	231	WP_000494241.1	YncJ family protein	-
G6Z98_RS08815	1841439..1843400	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
G6Z98_RS08820	1843473..1844009	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
G6Z98_RS08825	1844062..1845273	+	1212	WP_023143671.1	BenE family transporter YdcO	-
G6Z98_RS08830	1845381..1845839	-	459	WP_000526135.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1845381..1845839	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T150937 WP_001306887.1 NZ_CP049197:c1840895-1840719 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T150937 NZ_CP065152:c122482-122171 [Escherichia coli O150:H6]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT150937 WP_001270286.1 NZ_CP049197:c1840673-1840257 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT150937 NZ_CP065152:c122174-121755 [Escherichia coli O150:H6]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76