Detailed information of TA system TA150936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1659119..1659645
• Replicon: chromosome
• Accession: NZ_CP049197
• Organism: Escherichia coli strain E597

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: G6Z98_RS07925
• Protein ID: WP_000323025.1
• Coordinates: 1659358..1659645 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: G6Z98_RS07920
• Protein ID: WP_000534858.1
• Coordinates: 1659119..1659358 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z98_RS07895	1654381..1654995	+	615	WP_001265627.1	hypothetical protein	-
G6Z98_RS07900	1654992..1656020	+	1029	WP_001345551.1	DUF4868 domain-containing protein	-
G6Z98_RS07905	1656504..1657817	+	1314	WP_000589005.1	ISNCY family transposase	-
G6Z98_RS07910	1658254..1658586	-	333	WP_001301033.1	protein FlxA	-
G6Z98_RS07915	1658789..1659094	-	306	WP_001326990.1	hypothetical protein	-
G6Z98_RS07920	1659119..1659358	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
G6Z98_RS07925	1659358..1659645	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
G6Z98_RS07930	1659717..1659872	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
G6Z98_RS07935	1660089..1660340	+	252	WP_000980994.1	hypothetical protein	-
G6Z98_RS07940	1660407..1660685	+	279	WP_012304870.1	hypothetical protein	-
G6Z98_RS07945	1660687..1661736	+	1050	WP_001265199.1	DUF968 domain-containing protein	-
G6Z98_RS07950	1661750..1662502	+	753	WP_001047135.1	antitermination protein	-
G6Z98_RS07955	1662780..1662869	-	90	WP_120795389.1	hypothetical protein	-
G6Z98_RS07960	1662924..1663136	-	213	WP_000087756.1	cold shock-like protein CspF	-
G6Z98_RS07965	1663437..1663652	+	216	WP_000066484.1	cold shock-like protein CspB	-
G6Z98_RS07970	1664406..1664621	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1639569..1695252	55683

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T150936 WP_000323025.1 NZ_CP049197:1659358-1659645 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T150936 NZ_CP065152:67373-67693 [Escherichia coli O150:H6]
ATGCACATTTTATCTGTACCGACCACGGTGCCAGTTTCGTCACGCCTGTCGCCCGTACAGGTCTGGCAGCAACTGTTAAC
GTATCTGCTGGAGCAGCATTACGGCCTCACACTCAACGACACGCCATTCCACGACGACGCGGCTATCAAAGAACATATCG
AGGCGGGAATAACACTTGCTGATGCGGTGAATTTCCTGGTCGAAAAATACGAACTGGTTCGTATCGACCGAAAGGGGTTT
TCATGGCAGGATCAAACGCCATTCCTCAGCGCTACCGATATTCTCAGAGCAAGGCGAGCTACGGGGATTATGAGCACTTA
A

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT150936 WP_000534858.1 NZ_CP049197:1659119-1659358 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT150936 NZ_CP065152:67021-67341 [Escherichia coli O150:H6]
ATGAAAAATTCATGGGGACTGAGGCGCGACATGACGCCGTGCTTCGGTGCAAGGCTTGTGCAGGAAGGCCGTCGCCTGCA
TTTTCTGGCGGACCGTGCAGATATTAGCGGTGCGTTTAATGAGGAGGAGGCGCTACGTCTGGATCAAGCATTTCCGCTAG
TGATGAAACAGCTTGAATTGATGCTGACAACCGGTGAACTAAATCCACGCCATCAGCATTGCGTCACGTTGCATCATAAC
GGACTCACCTGCGAGGCGGATACTCTCGGCTCTCATGGCTACGTTTACATCGTTATTTATCCTCAGACATCGAGCCAGTA
A

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8