Detailed information of TA system TA150910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2532808..2533592
• Replicon: chromosome
• Accession: NZ_CP049196
• Organism: Escherichia coli strain E118

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: G5T40_RS12405
• Protein ID: WP_000613626.1
• Coordinates: 2533098..2533592 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: G5T40_RS12400
• Protein ID: WP_001110447.1
• Coordinates: 2532808..2533101 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5T40_RS12390	2527958..2528917	-	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
G5T40_RS12395	2529490..2532675	+	3186	WP_001522867.1	ribonuclease E	-
G5T40_RS12400	2532808..2533101	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
G5T40_RS12405	2533098..2533592	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
G5T40_RS12410	2533687..2534640	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
G5T40_RS12415	2534652..2536295	-	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
G5T40_RS12420	2536361..2537302	-	942	WP_001522863.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
G5T40_RS12425	2537302..2538399	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T150910 WP_000613626.1 NZ_CP049196:2533098-2533592 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T150910 NZ_CP065145:c1657625-1657242 [Proteus mirabilis]
ATGATATGGGTTAGCGCACAGGAAGTTATCGCTTTCCATGATCGTATATTACAACGTTTCCCAGGTGTGGCCGGTATGTC
CGATCCTGGCAGAGCGGAAGCTCTTATTTACCGGGTGCAAAACCGTAAACATTATGAAGGCATTACAGATGTGTTTGAAC
TGGCAGCAACTTATTGGGTCGCTATCGCTCGAGGGCACATATTTAATGACGGTAATAAACGTACCGCATTTTTTGTGACC
ATGACATTTCTCTATCGTAATGGCATCAGGATCCGCGATACTGGCAATATGCTGGAAAACTTAACAGTAGAAGCAGCTAC
AGGTGAGAAAACCGTCGACCAGTTAGCAAAACATCTACAAAACTTGGTAGAGAAAACTAACTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT150910 WP_001110447.1 NZ_CP049196:2532808-2533101 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT150910 NZ_CP065145:c1657843-1657622 [Proteus mirabilis]
ATGCGTACATATACCTCGACACAAGCCCGAGCCAATATTTCAGAAGTATTAGATATTGCGACCCACGGTGAACCTGTTGA
AATCACCCGCAGAGACGGTTGTTCAGCGGTTGTTATCAGTAAAACTGAATTTGAGTCATACCAGAATGCTAAGCTAGATG
CAGAGTTTGATATGATGATGCAACGTCATGGACATACCGTAGAGGCGCTGACAAATCGATGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6