Detailed information of TA system TA150908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2043949..2044587
• Replicon: chromosome
• Accession: NZ_CP049196
• Organism: Escherichia coli strain E118

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: G5T40_RS09810
• Protein ID: WP_001306887.1
• Coordinates: 2044411..2044587 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: G5T40_RS09805
• Protein ID: WP_001270286.1
• Coordinates: 2043949..2044365 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5T40_RS09785	2039101..2040042	-	942	WP_001251315.1	ABC transporter permease	-
G5T40_RS09790	2040043..2041056	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
G5T40_RS09795	2041074..2042219	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
G5T40_RS09800	2042464..2043870	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
G5T40_RS09805	2043949..2044365	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
G5T40_RS09810	2044411..2044587	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
G5T40_RS09815	2044809..2045039	+	231	WP_000494241.1	YncJ family protein	-
G5T40_RS09820	2045131..2047092	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
G5T40_RS09825	2047165..2047701	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
G5T40_RS09830	2047754..2048965	+	1212	WP_023143671.1	BenE family transporter YdcO	-
G5T40_RS09835	2049073..2049531	-	459	WP_000526135.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2049073..2049531	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T150908 WP_001306887.1 NZ_CP049196:c2044587-2044411 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T150908 NZ_CP065145:1298070-1298456 [Proteus mirabilis]
ATGGTTATTTATAAAACAAAGATGTTCAAAAATAGTTTCAAAAAAATAACGCTTACGAATGCAGAACTAATAGCTATCGC
TACCGAAGTCCTTGCAGGACAATTTGAGGCAGATCTAGGTGGTGGTGTTATCAAGAAACGTGCCTGTATCCAAGGAAAAG
GTAAAAGTAGCGGAATTAGAACAATTATATTTTATAAGCAAGGGAATAATTTATTCTTTGCTGATGGTTGGAAAAAATCG
AGTTTAAGCTCTAAAAAAACCAAAGAGATCACAGATGATGAACTTGAGTCCTATAAAGATCTAGCAAAAGATCTCTTCAA
TGCAAATCAAAATAAAATAGATAAAATGATTGCACTAGGCCTTTTAACAGAGGTTAAATATGACTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT150908 WP_001270286.1 NZ_CP049196:c2044365-2043949 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT150908 NZ_CP065145:1298449-1298769 [Proteus mirabilis]
ATGACTGATAAAATGAAAGAAATGCTAAAATTTGCACAAGAAATGGAAGATGCAGGAGTCACTGATGGCAGTACAACTAA
ACGAATTGCCGCCAGAATAAAAGCGAAAGAATTACAAGCTAAGTATAAACATCCTACCCCTATGACAGGTGAAGATATAA
AAAGGATCCGTGAGCAAAATAATATCTCTCAATCTTCCCTTGCTTTGATATTGAATATGACTAAAGAAAGCGTTTCTAAG
TGGGAAAGAAACGAAATTAAGCCCAATGGTGCAGCACTACGACTTCTGCATTTAATAGATAAAAAAATATTATTCATCTA
G

Structures

Toxin

• AlphaFold DB: A0A0D8WF76