Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1862812..1863338 Replicon chromosome
Accession NZ_CP049196
Organism Escherichia coli strain E118

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag G5T40_RS08925 Protein ID WP_000323025.1
Coordinates 1863051..1863338 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag G5T40_RS08920 Protein ID WP_000534858.1
Coordinates 1862812..1863051 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5T40_RS08895 1858074..1858688 + 615 WP_001265627.1 hypothetical protein -
G5T40_RS08900 1858685..1859713 + 1029 WP_001345551.1 DUF4868 domain-containing protein -
G5T40_RS08905 1860197..1861510 + 1314 WP_000589005.1 ISNCY family transposase -
G5T40_RS08910 1861947..1862279 - 333 WP_001301033.1 protein FlxA -
G5T40_RS08915 1862482..1862787 - 306 WP_001326990.1 hypothetical protein -
G5T40_RS08920 1862812..1863051 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
G5T40_RS08925 1863051..1863338 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
G5T40_RS08930 1863410..1863565 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
G5T40_RS08935 1863782..1864033 + 252 WP_000980994.1 hypothetical protein -
G5T40_RS08940 1864100..1864378 + 279 WP_012304870.1 hypothetical protein -
G5T40_RS08945 1864380..1865429 + 1050 WP_001265199.1 DUF968 domain-containing protein -
G5T40_RS08950 1865443..1866195 + 753 WP_001047135.1 antitermination protein -
G5T40_RS08955 1866473..1866562 - 90 WP_120795389.1 hypothetical protein -
G5T40_RS08960 1866617..1866829 - 213 WP_000087756.1 cold shock-like protein CspF -
G5T40_RS08965 1867130..1867345 + 216 WP_000066484.1 cold shock-like protein CspB -
G5T40_RS08970 1868099..1868314 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1848294..1900733 52439


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T150907 WP_000323025.1 NZ_CP049196:1863051-1863338 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T150907 NZ_CP065144:c3867458-3866145 [Proteus mirabilis]
ATGGCGAGTCTTGATGTATATATGAATGAATACTATGTGGGCATCTTTACTAAAAAAAGCTCAGGTGCGCATTCATTCCA
ATATACTGAAGATTGGGTTAATCAACCAGGAAGTCGTCCCATCTCTCTTTCGATGCCATTACAGCTTCAAGAATATCAAG
GAGACTGTGTCTATAATTTCTTTGATAATCTGTTACCTGATAATACTGAAATTAGAAATCGTATTGTGAGTCGCTATAAT
GCGAACTCAAATCAACCCTTTGACCTATTAGCGGCTATCGGTGCAGATACTGTTGGTGCATTAAGGTTACTTCCTAGTGG
CAGTACGCCTGCGGATATCAAAAAAATAGAATATCAAAAACTCAATGATACTGAGCTTGAAAAAATTCTACTAGGATATA
AATCCAATACTCCACTCGGTATGCTTAAAGAATATACTGATTTCAGAATTTCCATCGCTGGAGCACAAGAAAAAACGGCA
TTACTTTTAAAAGATAATCATTGGTGTGTGCCACATAATAACACACCAACTACCCATATTATTAAACTGCCAATTGGTGT
TATTGAAAGTCATTCTTATACAATGGATTTATCCAATAGTGTAGAAAATGAGTATCTTTGTACCTTAATTGCCAAAGAAT
TTGATCTTCCCGTCCCCCACTGCTCTATTATTAAAACAAAAAATGTTAAAGCGCTTGCTGTAGAACGTTTTGATAGAAAA
TATTCATCTGATAAATCGTGGATAATACGTTTACCTCAAGAAGATTTTTGCCAAATTTTAAATATACCTTCTGCGCTAAA
ATATGAAAATGAAGGAGGGCCTGGCATTATCGATATTATGAAATATTTACTTGGTTCATCAAATGCCGATCAAGATCGTT
TTCATTTTATGAAGGCACAAGTGCTATTTTGGCTTTTGGCTGCAACGGATGGACACGCAAAGAATTTTTCTGTTTTTATC
AACCAAGAAGGACGTTTCCATCTCACTCCCCTTTATGACATTATGTCCATCTATCCCAATTTCGGAGGACGAGGACTTAA
TCCACGAGATGCAAAACTTGCTATGGGATTAAAAGCAAGCCGAGGCAAAAAATACGCAATAAAACAAATTTTTCCTCGTC
ATTTCTACCAAACAGCTGAAGCTGTCGGTTTCGATCCTTCATTAATGAGCGATATTCTTCGTTATTTTTATCAAGAAATG
GACGATGTCATACAACGCGTAAAACAGCAATTACCCGAGGATTTCCCTTCTGAGATTAGCAATACAATATTAGATGGAAT
GAAAGCACGCGCTGTTAGATTAGCTAACATTTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT150907 WP_000534858.1 NZ_CP049196:1862812-1863051 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT150907 NZ_CP065144:c3867716-3867459 [Proteus mirabilis]
ATGATCATAATTAATGCCTATCAACTCAGTGTGCATCTAAAAGATGTCCGATTAACAAAAAAACTGTCTCAAAGTAAAGT
CGCCCAAAAAGTAGGGATCCGACAAGATACCGTGTCCAATTTTGAACTTAATCCTAATTCCACCAAACTAGAAACCTTTT
TCAAACTGCTCTCCGCACTAAATTTAGAGATGGAAATTCATCCTAAAGATGCTAAAAATAATTTAGCACACAAAAGTGAA
TGGAAAGAGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References