Detailed information of TA system TA150891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 87874..89497
• Replicon: plasmid pIHI2-2152
• Accession: NZ_CP049193
• Organism: Enterobacter hormaechei strain Y2152

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C1IUI3
• Locus tag: G5577_RS23000
• Protein ID: WP_000088044.1
• Coordinates: 88172..89497 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: G5577_RS22995
• Protein ID: WP_012477377.1
• Coordinates: 87874..88167 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5577_RS22975	83673..84065	+	393	WP_000341066.1	hypothetical protein	-
G5577_RS22980	84724..85380	+	657	WP_000462754.1	trypsin-like serine protease	-
G5577_RS22985	85583..86080	+	498	WP_000340139.1	membrane protein	-
G5577_RS22990	86085..87473	+	1389	WP_164474672.1	hypothetical protein	-
G5577_RS22995	87874..88167	+	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
G5577_RS23000	88172..89497	+	1326	WP_000088044.1	type II toxin-antitoxin system HipA family toxin	Toxin
G5577_RS23005	89558..89764	+	207	WP_000134171.1	hypothetical protein	-
G5577_RS23010	89865..90275	+	411	WP_000985909.1	hypothetical protein	-
G5577_RS23015	90288..91103	+	816	WP_001641519.1	HNH endonuclease	-
G5577_RS23020	91356..91781	+	426	WP_000278471.1	hypothetical protein	-
G5577_RS23025	92330..92638	+	309	WP_001224686.1	hypothetical protein	-
G5577_RS23030	92654..93511	+	858	WP_000784387.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA2 / tet(D) / aph(3')-Ia / mcr-9 / aph(6)-Id / aph(3'')-Ib / dfrA19 / sul1 / blaDHA-1 / qnrB4 / qacE / ere(A) / aac(6')-IIc	-	1..303394	303394

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50333.32 Da
• Isoelectric Point: 6.5044

>T150891 WP_000088044.1 NZ_CP049193:88172-89497 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T150891 NZ_CP065136:3944706-3944924 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT150891 WP_012477377.1 NZ_CP049193:87874-88167 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT150891 NZ_CP065136:3944306-3944680 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2S1XXW1

Antitoxin

• AlphaFold DB: A0A6M3PBR7