Detailed information of TA system TA150889

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3893056..3893723
• Replicon: chromosome
• Accession: NZ_CP049192
• Organism: Enterobacter hormaechei strain Y2152

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A1Z3MRP6
• Locus tag: G5577_RS18205
• Protein ID: WP_001581456.1
• Coordinates: 3893056..3893385 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: G5577_RS18210
• Protein ID: WP_000070396.1
• Coordinates: 3893406..3893723 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5577_RS18185	3888056..3889594	+	1539	WP_001581451.1	sugar ABC transporter ATP-binding protein	-
G5577_RS18190	3889597..3890679	+	1083	WP_006809988.1	ABC transporter permease	-
G5577_RS18195	3890768..3891718	+	951	WP_001581454.1	D-ribose ABC transporter substrate-binding protein	-
G5577_RS18200	3891849..3892706	+	858	WP_001581455.1	DeoR/GlpR family DNA-binding transcription regulator	-
G5577_RS18205	3893056..3893385	-	330	WP_001581456.1	toxin	Toxin
G5577_RS18210	3893406..3893723	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
G5577_RS18215	3893742..3893963	-	222	WP_000691994.1	DUF987 domain-containing protein	-
G5577_RS18220	3893972..3894448	-	477	WP_000811693.1	RadC family protein	-
G5577_RS18225	3894464..3894922	-	459	WP_000211838.1	antirestriction protein	-
G5577_RS18230	3895020..3895259	-	240	WP_000194654.1	DUF905 domain-containing protein	-
G5577_RS18235	3895336..3895803	-	468	WP_001581459.1	hypothetical protein	-
G5577_RS18240	3895827..3896270	-	444	WP_000824223.1	hypothetical protein	-
G5577_RS18245	3896270..3896506	-	237	WP_001144031.1	hypothetical protein	-
G5577_RS18250	3896547..3897248	-	702	WP_006812588.1	WYL domain-containing protein	-
G5577_RS18255	3897465..3898286	-	822	WP_000197389.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12456.52 Da
• Isoelectric Point: 8.5011

>T150889 WP_001581456.1 NZ_CP049192:c3893385-3893056 [Enterobacter hormaechei]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQTPYISVVDILRARRSTDLLKTNVK

Nucleotide

• Length: 330 bp

>T150889 NZ_CP065136:c3208167-3207793 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATATGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT150889 WP_000070396.1 NZ_CP049192:c3893723-3893406 [Enterobacter hormaechei]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT150889 NZ_CP065136:c3208588-3208214 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A1Z3MRP6

Antitoxin

• AlphaFold DB: S1EWR7