Detailed information of TA system TA150883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA(toxin)
• Location: 1259830..1261613
• Replicon: chromosome
• Accession: NZ_CP049192
• Organism: Enterobacter hormaechei strain Y2152

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: G5577_RS05980
• Protein ID: WP_045331150.1
• Coordinates: 1259830..1261179 (+)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A837FFV9
• Locus tag: G5577_RS05985
• Protein ID: WP_003859317.1
• Coordinates: 1261176..1261613 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5577_RS05955	1255653..1256588	-	936	WP_003859306.1	tRNA dihydrouridine(16) synthase DusC	-
G5577_RS05960	1256757..1257152	+	396	WP_003859308.1	CidA/LrgA family protein	-
G5577_RS05965	1257149..1257844	+	696	WP_003859310.1	CidB/LrgB family autolysis modulator	-
G5577_RS05970	1257973..1258857	+	885	WP_003859312.1	cytidine deaminase	-
G5577_RS05975	1258985..1259695	+	711	WP_003859314.1	outer membrane permeability protein SanA	-
G5577_RS05980	1259830..1261179	+	1350	WP_045331150.1	type II toxin-antitoxin system HipA family toxin	Toxin
G5577_RS05985	1261176..1261613	+	438	WP_003859317.1	helix-turn-helix domain-containing protein	Antitoxin
G5577_RS05990	1261652..1262662	-	1011	WP_003859318.1	galactose/methyl galactoside ABC transporter permease MglC	-
G5577_RS05995	1262678..1264198	-	1521	WP_003859320.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
G5577_RS06000	1264278..1265276	-	999	WP_164474519.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
G5577_RS06005	1265572..1266594	-	1023	WP_003859324.1	HTH-type transcriptional regulator GalS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (75-128)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 51140.83 Da
• Isoelectric Point: 4.6112

>T150883 WP_045331150.1 NZ_CP049192:1259830-1261179 [Enterobacter hormaechei]
METLTVQAYLNETWTDIALIKYPGSEKNDWNITQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRALINKITSSHMVQQQGYQFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Nucleotide

• Length: 1350 bp

>T150883 NZ_CP065136:2058130-2058384 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16185.64 Da
• Isoelectric Point: 10.5154

>AT150883 WP_003859317.1 NZ_CP049192:1261176-1261613 [Enterobacter hormaechei]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEEPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Nucleotide

• Length: 438 bp

>AT150883 NZ_CP065136:2057882-2058133 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A837FFV9