Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 141275..142898 Replicon plasmid pIHI2-323
Accession NZ_CP049189
Organism Enterobacter hormaechei strain Y323

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag G5C52_RS23370 Protein ID WP_000088044.1
Coordinates 141275..142600 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag G5C52_RS23375 Protein ID WP_012477377.1
Coordinates 142605..142898 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5C52_RS23340 137261..138118 - 858 WP_000784387.1 HNH endonuclease -
G5C52_RS23345 138134..138442 - 309 WP_001224686.1 hypothetical protein -
G5C52_RS23350 138991..139416 - 426 WP_000278471.1 hypothetical protein -
G5C52_RS23355 139669..140484 - 816 WP_001641519.1 HNH endonuclease -
G5C52_RS23360 140497..140907 - 411 WP_000985909.1 hypothetical protein -
G5C52_RS23365 141008..141214 - 207 WP_000134171.1 hypothetical protein -
G5C52_RS23370 141275..142600 - 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
G5C52_RS23375 142605..142898 - 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
G5C52_RS23380 143299..144687 - 1389 WP_000083579.1 hypothetical protein -
G5C52_RS23385 144692..145189 - 498 WP_000340139.1 membrane protein -
G5C52_RS23390 145392..146048 - 657 WP_000462754.1 trypsin-like serine protease -
G5C52_RS23395 146707..147099 - 393 WP_000341066.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 / aph(3')-Ia / tet(D) / catA2 / aac(6')-IIc / ere(A) / qacE / sul1 / qnrB4 / blaDHA-1 / blaSHV-12 / blaTEM-1B / aac(3)-IId / aadA2 / blaSFO-1 / dfrA12 - 1..394232 394232


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T150873 WP_000088044.1 NZ_CP049189:c142600-141275 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T150873 NZ_CP065136:219764-219871 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT150873 WP_012477377.1 NZ_CP049189:c142898-142605 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT150873 NZ_CP065136:c219715-219652 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References