Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 103596..104122 Replicon plasmid pIHI2-323
Accession NZ_CP049189
Organism Enterobacter hormaechei strain Y323

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag G5C52_RS23135 Protein ID WP_000323025.1
Coordinates 103596..103883 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag G5C52_RS23140 Protein ID WP_000534858.1
Coordinates 103883..104122 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5C52_RS23100 99166..99342 - 177 WP_001371930.1 hypothetical protein -
G5C52_RS23105 99853..100797 + 945 WP_000778029.1 DUF5417 domain-containing protein -
G5C52_RS23110 100893..101495 + 603 WP_012695474.1 hypothetical protein -
G5C52_RS23115 101555..101905 + 351 WP_000743059.1 hypothetical protein -
G5C52_RS23120 101952..102155 + 204 WP_001015183.1 hypothetical protein -
G5C52_RS23125 102437..102757 + 321 WP_000332796.1 hypothetical protein -
G5C52_RS23130 103366..103524 - 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
G5C52_RS23135 103596..103883 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
G5C52_RS23140 103883..104122 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
G5C52_RS23145 104385..105308 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
G5C52_RS23150 105508..106080 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
G5C52_RS23155 106556..107794 - 1239 WP_000219087.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 / aph(3')-Ia / tet(D) / catA2 / aac(6')-IIc / ere(A) / qacE / sul1 / qnrB4 / blaDHA-1 / blaSHV-12 / blaTEM-1B / aac(3)-IId / aadA2 / blaSFO-1 / dfrA12 - 1..394232 394232


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T150872 WP_000323025.1 NZ_CP049189:c103883-103596 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T150872 NZ_CP065136:219280-219387 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT150872 WP_000534858.1 NZ_CP049189:c104122-103883 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT150872 NZ_CP065136:c219222-219168 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References