Detailed information of TA system TA150857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 272784..273523
• Replicon: chromosome
• Accession: NZ_CP049188
• Organism: Enterobacter hormaechei strain Y323

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A3L9PBP9
• Locus tag: G5C52_RS01270
• Protein ID: WP_003857133.1
• Coordinates: 273038..273523 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A927HKN6
• Locus tag: G5C52_RS01265
• Protein ID: WP_045336276.1
• Coordinates: 272784..273050 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5C52_RS01245	268260..269246	+	987	WP_003857123.1	DUF979 domain-containing protein	-
G5C52_RS01250	269256..270251	+	996	WP_032658432.1	DUF2891 domain-containing protein	-
G5C52_RS01255	270273..271661	-	1389	WP_164474190.1	MFS transporter	-
G5C52_RS01260	271791..272720	+	930	WP_164474191.1	LysR family transcriptional regulator	-
G5C52_RS01265	272784..273050	+	267	WP_045336276.1	DUF1778 domain-containing protein	Antitoxin
G5C52_RS01270	273038..273523	+	486	WP_003857133.1	GNAT family N-acetyltransferase	Toxin
G5C52_RS01275	273825..274628	-	804	WP_045346200.1	carboxymuconolactone decarboxylase family protein	-
G5C52_RS01280	274644..275339	-	696	WP_207621942.1	antibiotic biosynthesis monooxygenase	-
G5C52_RS01285	275380..275985	-	606	WP_071957458.1	NAD(P)H-dependent oxidoreductase	-
G5C52_RS01290	276022..276399	-	378	WP_023292403.1	DUF1330 domain-containing protein	-
G5C52_RS01295	276438..277457	-	1020	WP_045346198.1	aldo/keto reductase	-
G5C52_RS01300	277460..278032	-	573	WP_045346197.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17540.23 Da
• Isoelectric Point: 9.9658

>T150857 WP_003857133.1 NZ_CP049188:273038-273523 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T150857 NZ_CP065052:4654-4941 [Acinetobacter baumannii]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTTGAAACTGCAAG
CCTTGTTTTTGAAGATCCTCTACGGATCTCAATCCAAGACAGACATACCAATGGGGAAGAGCGTTGGCAAACCATTGGTA
AGGTTAAAGGCGTACTGATGTTATTAGTCGCTCATACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTAACCAAAGCGGAGCGCAATCAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10032.46 Da
• Isoelectric Point: 4.4857

>AT150857 WP_045336276.1 NZ_CP049188:272784-273050 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWGV

Nucleotide

• Length: 267 bp

>AT150857 NZ_CP065052:4928-5239 [Acinetobacter baumannii]
ATGAGCATGGTTAGATATACACGCGAAGAGCTGAATGAAAATTTCAGCAACAAGCAAGATGCTGAAGTTGAACACTTGCT
TGCTAAGGGAACGGTTCCTGACGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGTAATGCTATTCGTC
ATGGGCAATTTTTTCGTCCAGTGAAGCAACAAACGTCAGTTCGTCTTGATGCGGATGTATTAGCATGGTTAAAAGCACAA
GGAAAAGGTTATCAAACACGTATGAATAAAATTCTGCGTGAAGCAATGCTAAAAGATTTAAAAAATCATTAA

Structures

Toxin

• AlphaFold DB: A0A3L9PBP9