Detailed information of TA system TA150851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4289388..4290225
• Replicon: chromosome
• Accession: NZ_CP049185
• Organism: Shigella sonnei strain 19.0821.3486

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: G6O60_RS21790
• Protein ID: WP_000227784.1
• Coordinates: 4289388..4289930 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: G6O60_RS21795
• Protein ID: WP_001297137.1
• Coordinates: 4289914..4290225 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O60_RS21760 (4284452)	4284452..4284943	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
G6O60_RS21765 (4285071)	4285071..4286435	-	1365	WP_001000971.1	MFS transporter	-
G6O60_RS21770 (4286554)	4286554..4287251	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
G6O60_RS21775 (4287620)	4287620..4288018	+	399	Protein_4242	SEL1-like repeat protein	-
G6O60_RS21785 (4288766)	4288766..4289332	+	567	Protein_4244	SEL1-like repeat protein	-
G6O60_RS21790 (4289388)	4289388..4289930	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
G6O60_RS21795 (4289914)	4289914..4290225	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
G6O60_RS21800 (4290410)	4290410..4291300	-	891	WP_000971327.1	heme o synthase	-
G6O60_RS21805 (4291312)	4291312..4291641	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
G6O60_RS21810 (4291641)	4291641..4292255	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
G6O60_RS21815 (4292245)	4292245..4294236	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
G6O60_RS21820 (4294258)	4294258..4295205	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T150851 WP_000227784.1 NZ_CP049185:c4289930-4289388 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T150851 NZ_CP065047:4937121-4937528 [Mycobacterium kubicae]
GTGATCTACCTGGATACCTCGGCTCTGGTCAAGCTGATCCGGATCGAAGTCGAATCTGATGCTCTAGCTGACTGGCTCGA
TGAACGCACCGAAACACGTTGGATAACATCTGCCTTGGCTGAAGTGGAGCTCCCCAGAGCCATCCGCGCAGTCGCCCCAA
ATGGCTTATCTGCTGTCCCCTCTGTGCTGGCCAGACTGGATCGGTTCGAAATCGACCAGGTCATCCGCGCCACCGCGGCC
GCCTACCCCGATCCGGCGCTGCGCTCGCTCGATGCGATACACCTGGCCACCGCTCAAATCGCCGCATCCACAGCACCATT
GACCGCCATGGTCGCCTATGACAGTCGACTCAGCGACGCCGCCCGTGCCCTCGGGATAACGATTGTCACACCTGGAAGAA
ACTCATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT150851 WP_001297137.1 NZ_CP049185:c4290225-4289914 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT150851 NZ_CP065047:4936845-4937117 [Mycobacterium kubicae]
ATGAGTGAAGTACCTCTCCGAATGCTCAACCAAGAGACGGCAAAGGTCTTGGCCCGCGTCAAGCGTGGCGAAGAGATCAC
GCTTACTGAGCGCGGGGAAGTCATCGCCCGAATAATCCCCGCTACCGCCGGCCCACTGGATGCGTTGATCACTGCCGGCA
GAGTGCAACCGGCGACGCTGGCCGGCCCAGCCCCGCGGCCGACCGTTCCGATGCACGACAATCTCGACGCCGGCGCGCTA
TTGCAACGTCTGCGCGCCGAGGAAAGGTATTAG

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3