Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2239051..2239705 Replicon chromosome
Accession NZ_CP049185
Organism Shigella sonnei strain 19.0821.3486

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag G6O60_RS11635 Protein ID WP_000244777.1
Coordinates 2239051..2239458 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G6O60_RS11640 Protein ID WP_000354046.1
Coordinates 2239439..2239705 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O60_RS11615 (2235008) 2235008..2236741 - 1734 WP_000813167.1 single-stranded-DNA-specific exonuclease RecJ -
G6O60_RS11620 (2236747) 2236747..2237457 - 711 WP_000715206.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G6O60_RS11625 (2237482) 2237482..2238378 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G6O60_RS11630 (2238490) 2238490..2239011 + 522 WP_001055874.1 flavodoxin FldB -
G6O60_RS11635 (2239051) 2239051..2239458 - 408 WP_000244777.1 protein YgfX Toxin
G6O60_RS11640 (2239439) 2239439..2239705 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G6O60_RS11645 (2239948) 2239948..2240928 + 981 WP_000886060.1 tRNA-modifying protein YgfZ -
G6O60_RS11650 (2241124) 2241124..2241783 - 660 WP_000250274.1 hemolysin III family protein -
G6O60_RS11655 (2241947) 2241947..2242258 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
G6O60_RS11660 (2242303) 2242303..2243736 + 1434 WP_005137189.1 6-phospho-beta-glucosidase BglA -
G6O60_RS11665 (2243793) 2243793..2244536 - 744 WP_000951937.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T150847 WP_000244777.1 NZ_CP049185:c2239458-2239051 [Shigella sonnei]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T150847 NZ_CP065044:c4555782-4555495 [Pectobacterium aroidearum]
ATGACCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAATTAGGGCATCCTGTCAGAGAACAATTCAA
AAAAAAGCTAGTTGAGCGTTTAGAAAATCCTCGTGTTCCTTCAGCCCAGCTCCATGGTCGTAAGGACCAGTATAAAATTA
AACTGAAGTCTGCTGGTTACAGGCTGGTGTATTTGGTTCAGGATGAGACCATTACGGTGATGGTCATGGGGGTTGGAAAG
CGCGAAGGTAGTCAGGTTTATTCTGATACTAAAGGTCGCTCTGCTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT150847 WP_000354046.1 NZ_CP049185:c2239705-2239439 [Shigella sonnei]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT150847 NZ_CP065044:c4556026-4555772 [Pectobacterium aroidearum]
ATGGCTTTCAATATCCTAACTAACACAGCGGCCAGTATCACTGAACTGAAACGCGACCCAATGGGGACAGTACGGGCTGC
TGAAGGTGAGACTATCGCTATACTGAACCGGAATGAGCCTGCGTTCTATTGTGTGCCTCCCGCCATGTTCGCTTATTTAA
TGGAATTGGCTGAAGATGCAGAGCTGGGGCGTACTGTTGATGATCGTATGTCAGAGTTAGCTGACGCGGAAGAGATTTCG
CTCGATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References