Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2330462..2331116 Replicon chromosome
Accession NZ_CP049179
Organism Shigella sonnei strain L4094

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag G6O64_RS11510 Protein ID WP_000244777.1
Coordinates 2330709..2331116 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G6O64_RS11505 Protein ID WP_000354046.1
Coordinates 2330462..2330728 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O64_RS11480 (2325631) 2325631..2326374 + 744 WP_000951937.1 SDR family oxidoreductase -
G6O64_RS11485 (2326431) 2326431..2327864 - 1434 WP_005137189.1 6-phospho-beta-glucosidase BglA -
G6O64_RS11490 (2327909) 2327909..2328220 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
G6O64_RS11495 (2328384) 2328384..2329043 + 660 WP_000250274.1 hemolysin III family protein -
G6O64_RS11500 (2329239) 2329239..2330219 - 981 WP_000886060.1 tRNA-modifying protein YgfZ -
G6O64_RS11505 (2330462) 2330462..2330728 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G6O64_RS11510 (2330709) 2330709..2331116 + 408 WP_000244777.1 protein YgfX Toxin
G6O64_RS11515 (2331156) 2331156..2331677 - 522 WP_001055874.1 flavodoxin FldB -
G6O64_RS11520 (2331789) 2331789..2332685 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G6O64_RS11525 (2332710) 2332710..2333420 + 711 WP_000715206.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G6O64_RS11530 (2333426) 2333426..2335159 + 1734 WP_000813167.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T150818 WP_000244777.1 NZ_CP049179:2330709-2331116 [Shigella sonnei]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T150818 NZ_CP065038:94650-94928 [Leifsonia sp. ZF2019]
GTGAAGGACCCGAGCTACCGCATCGAGCTGTCTCGGCCAGCGGCGCGCGCCCTGGCTCGGTTGACGCCGAAGATGGCGGA
CGCCGTGTTGCGGTTCCTGGATGGACCACTGTCGGACAACCCGATGCGAGTGACGAAGCCGTTGGGTGCCGAGCTGCAGG
GGATGCGGTCTGGCTACGTGGGCATCGCTTACCGGGTGCTGGTTCGCGTCGACGAAGAACAGCGATTGGTTGAGGTGATG
CGGATCGCGCATCGCTCAGATGCCTACCGCGGCTTCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT150818 WP_000354046.1 NZ_CP049179:2330462-2330728 [Shigella sonnei]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT150818 NZ_CP065038:94384-94653 [Leifsonia sp. ZF2019]
ATGCAGACGATGTCGGTGAGTGAAGCGAAGGACAAGCTGTCGAACCTGGTTGAGGGTGTCGAAGCGACGCATGACGCCGT
GGTGATCACGCGCCATGGGAAGCCGGCCGCCGTGCTGATCTCTCCCGAGGATCTGGATGCCCTGCAGGAGACGCTGGCCT
GGCTGTCTGATCCGTCGCACGCGGCCGAGATGGCTGAAGCTGAGGAGGATGCTGCCGAGGGGCGGATGCTGTCCTTGGAT
GAGGTTCGAGCGCAACTGGCGAATCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References