Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 963522..964019 Replicon chromosome
Accession NZ_CP049179
Organism Shigella sonnei strain L4094

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G6O64_RS04720 Protein ID WP_001173343.1
Coordinates 963522..963695 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag G6O64_RS04725 Protein ID WP_000398619.1
Coordinates 963723..964019 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O64_RS04700 (958674) 958674..959830 + 1157 WP_112368105.1 IS3-like element IS600 family transposase -
G6O64_RS04705 (959876) 959876..960133 + 258 Protein_916 lysine decarboxylase LdcC -
G6O64_RS04710 (960192) 960192..961649 + 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
G6O64_RS04715 (961886) 961886..963403 + 1518 WP_001295086.1 lysine--tRNA ligase -
G6O64_RS04720 (963522) 963522..963695 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G6O64_RS04725 (963723) 963723..964019 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G6O64_RS04730 (964246) 964246..964518 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
G6O64_RS04735 (964530) 964530..964760 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
G6O64_RS04740 (964941) 964941..966572 + 1632 WP_001216495.1 sensor histidine kinase -
G6O64_RS04745 (966569) 966569..967288 + 720 WP_000611285.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T150816 WP_001173343.1 NZ_CP049179:c963695-963522 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T150816 NZ_CP065037:4124331-4124582 [Leifsonia sp. ZF2019]
ATGAAGACGCTCAGCTATTCGGAGTCGCGCGCGCACTATGCCGCTACGCTCGACGCCGTGCTCGACGACCGCGAGGAGGT
CGTCATCACCCGCGCCGGGCGGGAGCCTGTGGTGATGGTCGCGCTCGAGGACTACGAGTCGCTCAAGGAGACCGCCTACC
TGCTGCGTAGCCCGGCCAACGCGCGCCGCCTGATCGAGTCGATCGAACGTCTCGATGACGGCGAAGGCACCCCGCACGAT
CTGCTCGAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT150816 WP_000398619.1 NZ_CP049179:c964019-963723 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT150816 NZ_CP065037:4124579-4124839 [Leifsonia sp. ZF2019]
ATGAAGCTGATCTGGGACCAGAACGCCTGGGAGGACTACCTCTACTGGCAGGCGCAGGACCGTCGCGTAGTGAAGCGCAT
CAACCAGCTCCTGGTGGACATCCTGCGCAACGGCAATACCGGGATCGGCAAGCCCGAGGCGCTCAAGCACGGGTTCCAGG
GGTTCTGGTCGCGGCGCATCACGGATGAGCATCGGCTCGTCTACAAGGTGACGGAGGATGCGGTGCTGATCGCCCAGTGT
CGGTACCACTACGCCCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References