Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 187981..188478 Replicon chromosome
Accession NZ_CP049177
Organism Shigella sonnei strain 1205.3131

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G6O63_RS00865 Protein ID WP_001173343.1
Coordinates 188305..188478 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag G6O63_RS00860 Protein ID WP_000398619.1
Coordinates 187981..188277 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O63_RS00840 (184712) 184712..185431 - 720 WP_000611285.1 two-component system response regulator DcuR -
G6O63_RS00845 (185428) 185428..187059 - 1632 WP_001216495.1 sensor histidine kinase -
G6O63_RS00850 (187240) 187240..187470 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
G6O63_RS00855 (187482) 187482..187754 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
G6O63_RS00860 (187981) 187981..188277 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G6O63_RS00865 (188305) 188305..188478 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G6O63_RS00870 (188597) 188597..190114 - 1518 WP_001295086.1 lysine--tRNA ligase -
G6O63_RS00875 (190351) 190351..191808 - 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
G6O63_RS00880 (191867) 191867..192124 - 258 Protein_164 lysine decarboxylase LdcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T150790 WP_001173343.1 NZ_CP049177:188305-188478 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T150790 NZ_CP065032:4541027-4541377 [Serratia sp. CMO1]
ATGGTGAAGCGCCCCGTTTTTCAGCGCGGAGATCTTATCCGGGTCAGCCTGAATCCCGTGGTTGGCCGTGAGCAGCAAGG
CGACTTTCGTCCGGCACTGGTGCTCTCTCCCCGCGAGTTTAATGCGTTGGGCATGGTGCTGGTCGCCCCTATTTCGCAAG
GGGCTAATTTTGCCCGTACAGCGGGTTTCACCGTCAGCCTGAGCGGGAGCGGCACCGCAACGCAGGGCGTCATCTTGATC
AACCAGGTTCGCATGATGGATCTGGCCGGGCGCAGCGCGCAATTCATTGAACGTGCCCCTGGCGAAGTCATCGCCGACGC
GCTGGCGCGGCTTCAGGCAATCATCGACTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT150790 WP_000398619.1 NZ_CP049177:187981-188277 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT150790 NZ_CP065032:4540785-4541033 [Serratia sp. CMO1]
ATGAAAGTCTTTATCAAAAAATGGGGCAACAGCGCGGGAATGCTGGTGCCCTCAGCGCTAATGAACGAGCTGGAACTACA
GGTAGGTCAGGAAATGGACATGCAGGTCATCGATGGAGCGTTGGTCATTAAACCGGTTAAGAAACGCTATTCACTGCAGC
AATTGCTCGATGGCTGCGACGGTGAAGCGCCGGCCGCCGCAGACGACAACTGGGAACACATGCCGCCGGCGGGGAAAGAA
GTATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References