Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1872623..1873120 Replicon chromosome
Accession NZ_CP049175
Organism Shigella sonnei strain 7111.69

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G6O62_RS09480 Protein ID WP_001173343.1
Coordinates 1872623..1872796 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag G6O62_RS09485 Protein ID WP_000398619.1
Coordinates 1872824..1873120 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O62_RS09460 (1867775) 1867775..1868931 + 1157 WP_112368105.1 IS3-like element IS600 family transposase -
G6O62_RS09465 (1868977) 1868977..1869234 + 258 Protein_1847 lysine decarboxylase LdcC -
G6O62_RS09470 (1869293) 1869293..1870750 + 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
G6O62_RS09475 (1870987) 1870987..1872504 + 1518 WP_001295086.1 lysine--tRNA ligase -
G6O62_RS09480 (1872623) 1872623..1872796 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G6O62_RS09485 (1872824) 1872824..1873120 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G6O62_RS09490 (1873347) 1873347..1873619 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
G6O62_RS09495 (1873631) 1873631..1873861 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
G6O62_RS09500 (1874042) 1874042..1875673 + 1632 WP_001216495.1 sensor histidine kinase -
G6O62_RS09505 (1875670) 1875670..1876389 + 720 WP_000611285.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T150779 WP_001173343.1 NZ_CP049175:c1872796-1872623 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T150779 NZ_CP065031:4901881-4902168 [Pectobacterium brasiliense]
ATGAGCTATAGCGTTAAATTCAGGGAAGATGCGCTGAAAGAATGGCTCAAGCTCGATAAAACCATCCAGCAGCAATTTGC
CAAAAAACTAAAAAAATGCTGCGAAAATCCCCATATTCCTTCAGCAAAGTTACGGGGGATGAAGGACTGCTACAAAATAA
AGCTCCGAGCGTCAGGCTTTCGTTTGGTTTATGAGGTTATTGACGACGTATTGATTATCGCCGTAGTGGCGGTAGGTAAA
CGTGAGCGCAGCGGTGTTTATCATCTGGCGAGTGAGCGAATGAGATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT150779 WP_000398619.1 NZ_CP049175:c1873120-1872824 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT150779 NZ_CP065031:4901633-4901884 [Pectobacterium brasiliense]
ATGCCGAATATTATATTGAGTGATACCAGTGCCAGCGTCAGTGAACTGAAAAAGAACCCGATGGCGACGGTCAATGCTGG
CGATGGCCTGCCTGTTGCTATCTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGATCTCTACGAAAAAATGC
TGGATGCGCTGGACGATCAAGAACTGGTTAAGCTAATCGGTGAACGCAAAAACCAGCCGCTGGTTGACGTGGATTTGGAT
AGTTTCCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References