Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3165232..3165729 Replicon chromosome
Accession NZ_CP049173
Organism Shigella sonnei strain 19.0820.1561

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G6O61_RS16150 Protein ID WP_001173343.1
Coordinates 3165232..3165405 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag G6O61_RS16155 Protein ID WP_000398619.1
Coordinates 3165433..3165729 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O61_RS16130 (3160384) 3160384..3161540 + 1157 WP_087879933.1 IS3-like element IS600 family transposase -
G6O61_RS16135 (3161586) 3161586..3161843 + 258 Protein_3150 lysine decarboxylase LdcC -
G6O61_RS16140 (3161902) 3161902..3163359 + 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
G6O61_RS16145 (3163596) 3163596..3165113 + 1518 WP_001295086.1 lysine--tRNA ligase -
G6O61_RS16150 (3165232) 3165232..3165405 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G6O61_RS16155 (3165433) 3165433..3165729 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G6O61_RS16160 (3165956) 3165956..3166228 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
G6O61_RS16165 (3166240) 3166240..3166470 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
G6O61_RS16170 (3166651) 3166651..3168282 + 1632 WP_001216495.1 sensor histidine kinase -
G6O61_RS16175 (3168279) 3168279..3168998 + 720 WP_000611285.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T150760 WP_001173343.1 NZ_CP049173:c3165405-3165232 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T150760 NZ_CP065030:c3002969-3002649 [Pectobacterium versatile]
ATGCAAACATCACCTGCAATCCCGCCACGGGAGGATATCCCTTGCCCGTCACCTATAGCCGTCTGGCAGCAACTGCTTAC
CTACCTGCTGGAAAAGCACTACGGCCTGACACTGAACGACACACCGTTCTGTGAAGAAAACGTGATTCAGGAACATATTG
ATGCCAGTGTCACTTTGGTCAATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATTGACCGCAACGGCTTT
AACTGGCAGGAACAATCGCCGTTTCTTACCGCTGTTGATATTCTCCGTGCCAGACGCGCTACCGGCTTGCTCAAAGCATA
A

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT150760 WP_000398619.1 NZ_CP049173:c3165729-3165433 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT150760 NZ_CP065030:c3003373-3003038 [Pectobacterium versatile]
ATGCCATCATCGGATACCCCAGAATGGGGATTAAAATGTGCCGTCACGCCGCGATTCGGAGCCAGACTGGTTCAGGAAGG
TAACCGTCTGCATTATCTGGCAGACCGGGCCAGTTTTATCGGTATGTTCAGCAAAACAGAAGCCCGAAATCTGGAGCGTT
GCTTCTCTGAGTTGATTAAGCAGTTGGAACAGAAACTGTGCACGGGTGAGCTGAATCCCCGCCAGCAAGGGTGTGTCACG
CTGCATTGTGACGAATTTACCTGTGAAGCCGATACGCTGGGCAGTTTTGGCTACGTGTATATTGTTATCTATCCCTCTTC
GGCAGCAGCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References