Detailed information of TA system TA150716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3743574..3744058
• Replicon: chromosome
• Accession: NZ_CP049169
• Organism: Shigella sonnei strain 19.1125.3493

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A377EAU1
• Locus tag: G6O58_RS18720
• Protein ID: WP_014334020.1
• Coordinates: 3743768..3744058 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A376K4R9
• Locus tag: G6O58_RS18715
• Protein ID: WP_005142544.1
• Coordinates: 3743574..3743768 (+)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O58_RS18675 (3738769)	3738769..3739125	-	357	WP_005140877.1	hypothetical protein	-
G6O58_RS18680 (3739183)	3739183..3739605	-	423	WP_001151171.1	DUF977 family protein	-
G6O58_RS18685 (3739621)	3739621..3740346	-	726	WP_000450631.1	DUF1627 domain-containing protein	-
G6O58_RS18690 (3740368)	3740368..3741114	-	747	WP_000788740.1	ATP-binding protein	-
G6O58_RS18695 (3741121)	3741121..3742185	-	1065	WP_001262380.1	hypothetical protein	-
G6O58_RS18700 (3742257)	3742257..3742682	-	426	WP_000693826.1	toxin YdaT family protein	-
G6O58_RS18705 (3742679)	3742679..3742942	-	264	WP_001048456.1	helix-turn-helix transcriptional regulator	-
G6O58_RS18710 (3743069)	3743069..3743554	+	486	WP_053002468.1	helix-turn-helix domain-containing protein	-
G6O58_RS18715 (3743574)	3743574..3743768	+	195	WP_005142544.1	hypothetical protein	Antitoxin
G6O58_RS18720 (3743768)	3743768..3744058	+	291	WP_014334020.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G6O58_RS18725 (3744338)	3744338..3744490	+	153	WP_000379558.1	DUF1391 family protein	-
G6O58_RS18730 (3744502)	3744502..3744867	+	366	WP_024174355.1	hypothetical protein	-
G6O58_RS18740 (3746245)	3746245..3747033	+	789	Protein_3644	Rha family transcriptional regulator	-
G6O58_RS18750 (3747782)	3747782..3748858	+	1077	Protein_3646	molybdopterin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3725782..3747351	21569

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11062.93 Da
• Isoelectric Point: 10.2013

>T150716 WP_014334020.1 NZ_CP049169:3743768-3744058 [Shigella sonnei]
MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLTEHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIE
VVNIVHSRRQYPGKNS

Nucleotide

• Length: 291 bp

>T150716 NZ_CP064942:2031979-2032254 [Pontivivens sp. MT2928]
GTGCCAAGACTTGAATGGAAGATTGCCGCCGTCTCTGACCTCTTAGCAATCATCGACTACATCTCAGACGACAATCCGGA
TGCCGCGCAGGCGCTCAAGAACGAGATCGAAGAGAAAGTCTCCAGACTTCCCACGAACCCACACCTCTATCGCGTCGGTC
GTGTCGACGGCACGCGAGAGATGGTGGTCCGTCCGAACTACATTGTCGTGTATTCGGAGACCCCAGAGGTCGTGACCATC
CTACGTGTTCTTCACGCGGCACAGCTCTGGCCCTGA

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7097.98 Da
• Isoelectric Point: 4.1759

>AT150716 WP_005142544.1 NZ_CP049169:3743574-3743768 [Shigella sonnei]
MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDEVMAEMEQIIAQIAAATHRGE

Nucleotide

• Length: 195 bp

>AT150716 NZ_CP064942:2031693-2031992 [Pontivivens sp. MT2928]
ATGCCCGCCCAGACCTCCATGATCCACGTGCGTGTCGATGATCAGCTGAAGACCGATGCGTCCGAAGCCCTTGCAGGTGT
CGGTCTGACGGTCTCCGACGCTGTTCGTATATTGCTGACGCGCGTTGCTGCCGAGGGGGGGTTGCCCGCTGGACTGACTG
CTGATCCCGATGCCTACGACGCTTGGTTCCGGGCCAAGGTCCAGGAAGCGATTGACGATCCGCGCCCTCCCGCACCACAT
GCGCAAATCATGGCCGAAGCCCAGGCGCTGATTGACGGAAAGCGACGTGCCAAGACTTGA

Structures

Toxin

• AlphaFold DB: A0A377EAU1

Antitoxin

• AlphaFold DB: A0A376K4R9