Detailed information of TA system TA150701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4242349..4243186
• Replicon: chromosome
• Accession: NZ_CP049167
• Organism: Shigella sonnei strain 09163633

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: G6O57_RS21455
• Protein ID: WP_000227784.1
• Coordinates: 4242644..4243186 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: G6O57_RS21450
• Protein ID: WP_001297137.1
• Coordinates: 4242349..4242660 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O57_RS21425 (4237369)	4237369..4238316	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
G6O57_RS21430 (4238338)	4238338..4240329	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
G6O57_RS21435 (4240319)	4240319..4240933	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
G6O57_RS21440 (4240933)	4240933..4241262	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
G6O57_RS21445 (4241274)	4241274..4242164	+	891	WP_000971327.1	heme o synthase	-
G6O57_RS21450 (4242349)	4242349..4242660	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
G6O57_RS21455 (4242644)	4242644..4243186	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
G6O57_RS21460 (4243242)	4243242..4243808	-	567	Protein_4185	SEL1-like repeat protein	-
G6O57_RS21465 (4243842)	4243842..4244539	+	698	WP_094096600.1	IS1-like element IS1A family transposase	-
G6O57_RS21470 (4244556)	4244556..4244954	-	399	Protein_4187	SEL1-like repeat protein	-
G6O57_RS21480 (4246139)	4246139..4247503	+	1365	WP_001000971.1	MFS transporter	-
G6O57_RS21485 (4247631)	4247631..4248122	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4245323..4245589	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T150701 WP_000227784.1 NZ_CP049167:4242644-4243186 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T150701 NZ_CP064923:c4987203-4986784 [Acaryochloris marina S15]
GTGAGCCTTCGTGTGAAAGTTCTCATCGATACCAATGTTGTCCTAGATGTACTCCTGGAACGCGACCCCTTTGTCGAGAA
TGCAATTGCTCTCTTTGAGCAAATTGAACTCGGTCACGTAGTAGGTGCAATTGCTGCAACAACCCTGACCAATATTTTCT
ACATCATCCGTAAATCAGAAAGTCGTGAAGCTGCCATCTCTGCTGTCCATCGACTCCTGATCGGTCTCCAATTTTGTGCT
GTTGATCGCCAGACAGTCGAAACTGCTCTCGGTCTTGGGCTAAAAGACTTTGAAGACAGTATTCAACTTGCCTGCGCACT
CCTCAATCAATTGGATGGCATCGTTACCCGTGATTCAAAAGATTTCATTAGCAGCCAGCTTCCCATTTACTCCCCTACTG
AACTCTTAAGCCAGCTCTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT150701 WP_001297137.1 NZ_CP049167:4242349-4242660 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT150701 NZ_CP064923:c4987439-4987200 [Acaryochloris marina S15]
ATGACAGCCGCAGAAAAGCTCTACGAACTTATTCAATCTCTCCCAGAAAGCCAAGTCAACGAAGTCCTCAACTTCGCCGA
ATTTCTCCATCATAAGCAGCTCAATAGTTCTCAATCGCAGGCTATCCCGCCTGGGACGCTTACCGGACTGCGGGGTATCG
CCAAGGGGCCTAAAGCAGCTTCTAGCGATCATGAATTGCAAGCAGAATACACAGATTATCTCACCCAAAAATATCAGTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3