Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2960438..2960935 Replicon chromosome
Accession NZ_CP049165
Organism Shigella sonnei strain 0401952027

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G6O56_RS15080 Protein ID WP_001173343.1
Coordinates 2960438..2960611 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag G6O56_RS15085 Protein ID WP_000398619.1
Coordinates 2960639..2960935 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O56_RS15060 (2955590) 2955590..2956746 + 1157 WP_087879933.1 IS3-like element IS600 family transposase -
G6O56_RS15065 (2956792) 2956792..2957049 + 258 Protein_2946 lysine decarboxylase LdcC -
G6O56_RS15070 (2957108) 2957108..2958565 + 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
G6O56_RS15075 (2958802) 2958802..2960319 + 1518 Protein_2948 lysine--tRNA ligase -
G6O56_RS15080 (2960438) 2960438..2960611 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G6O56_RS15085 (2960639) 2960639..2960935 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G6O56_RS15090 (2961162) 2961162..2961434 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
G6O56_RS15095 (2961446) 2961446..2961676 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
G6O56_RS15100 (2961857) 2961857..2963488 + 1632 WP_001216495.1 sensor histidine kinase -
G6O56_RS15105 (2963485) 2963485..2964204 + 720 WP_000611285.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T150676 WP_001173343.1 NZ_CP049165:c2960611-2960438 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T150676 NZ_CP064879:c19942-19580 [Tolypothrix sp. PCC 7601]
ATGCAGAGTGATAACACGGTTTCAATTCGATTTGCTGATGAGTTTGAGGAGAAACTCTACAGACTCTCAAAAAGATTTCG
CAATATTCGCTCTGATGTTCAACCCATTATCGAGCAATTACAACAAGGAAATATTGTAGGAGACAGGATTAGCGGTCTGG
GTGAGGAGTATATTGTTTACAAGGTGAGAGTTCGCAACAGTAATATTCAAAAAGGTAAAAGCGGTGGATACCGCCTCATC
TATCAACTTGAGTCACCCACAAGTATTTTGCTATTAACGATTTATTCCAAATCCGACCAAGAAGACATTGCTGCAAATGA
AATCCGCAATATTCTAGCTGATTTTGATAGTGAGGAAGGATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT150676 WP_000398619.1 NZ_CP049165:c2960935-2960639 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT150676 NZ_CP064879:c20189-19932 [Tolypothrix sp. PCC 7601]
ATGAAAGCGATCGAAGTGACTGGTAAGATTGACGCTCAAGGCAACCTGATTCTAGATGAGCCAATTCACGGAACAACCTA
TCCTCATCAGGTGCGGGTAATTGTATTGGTTCCAGAACCAGCAGAAACAGAAGAACTTGACCCAGATGATACACCAGTTG
AGGAAATTAAAGCTAGCTTGAGGAGAGCCTTAAAGCAAGCAAAAATGGGACAAACTCGACCAATTAGCGAGTTATGGGAC
AGAATTGATGCAGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References