Detailed information of TA system TA150659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4422968..4424225
• Replicon: chromosome
• Accession: NZ_CP049161
• Organism: Pseudomonas aeruginosa strain MS14403

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: WPAWT_RS20315
• Protein ID: WP_023084953.1
• Coordinates: 4422968..4423723 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: WPAWT_RS20320
• Protein ID: WP_003086329.1
• Coordinates: 4423857..4424225 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WPAWT_RS20305	4418141..4420684	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
WPAWT_RS20310	4420839..4422200	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
WPAWT_RS20315	4422968..4423723	-	756	WP_023084953.1	RES family NAD+ phosphorylase	Toxin
WPAWT_RS20320	4423857..4424225	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
WPAWT_RS20325	4424298..4425584	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
WPAWT_RS20330	4425640..4427229	-	1590	WP_079384792.1	aldehyde dehydrogenase family protein	-
WPAWT_RS20335	4427307..4427783	+	477	WP_031675481.1	elongation factor GreAB	-
WPAWT_RS20340	4427786..4428061	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28310.49 Da
• Isoelectric Point: 7.7294

>T150659 WP_023084953.1 NZ_CP049161:c4423723-4422968 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGMRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T150659 NZ_CP064868:1145950-1146096 [Mammaliicoccus sciuri]
GTGAGAAATTTGAATAAAAATCTAGAAGAAGAAAATGAAATGGTTCAAGACCTAGAAGATGTTAAAGAACTAGGTAAAGA
AATGGAACAAATATCAGAACAAAATGATGAAGCTTTTGAAGAAAAGAAATCTGAACAAGAGTCTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT150659 WP_003086329.1 NZ_CP049161:c4424225-4423857 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT150659 NZ_CP064868:1145346-1145945 [Mammaliicoccus sciuri]
ATGGCTTTAAACTTTAAAGTATTTGAAACAAAAGATATTGCTGCACGATATGCTGCAGATGTAGTTCGCAAACAATTTAA
TTCAGATAGCACTTCAGTTGTCGGTGTTCATTTAGAAGCAGAACAAACATCATTTTTCGATGAACTATTAGATGATGTTA
AGAAAAATCCTGTGAACTTCAGTCAAATTCATATTTTAGACTTTGACGGTAAAGAAGATTATTACACAGAATTAGGTGTC
CCAGCTAAACAAGTATTAAAAACTGATGTCGGTAGTGATGTTGAAGCATTTATTAAAGAAAAAGTGAATACTGACAAAAA
GAAAAACAAATTGATGTTAGATGTTGTCACTTTAACTGAAGATAGCAGAACGGGATTAGACTATGATCAAGCAATTCACC
CAGCAAGAGAAGTTATCGTACTTGTCACTGGTAAAGAAAAAGCTAAAGCAGTAAGTCAGTTATATCAAGAACCTGCAAAT
GGTTCTGTTGAATCCGCTAAATTAAAACAACATAGAATGGTAACAGTTGTATTAGATAATGAAGCGGCACAAGGGTTACC
TGAAGACGTTAGATCTTACTTCTCAATTCAATTTTCATAA

Structures

Antitoxin

• AlphaFold DB: V6AJU7