Detailed information of TA system TA150658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3473398..3474619
• Replicon: chromosome
• Accession: NZ_CP049161
• Organism: Pseudomonas aeruginosa strain MS14403

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: WPAWT_RS15840
• Protein ID: WP_003110488.1
• Coordinates: 3474041..3474619 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: WPAWT_RS15835
• Protein ID: WP_003088112.1
• Coordinates: 3473398..3473952 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WPAWT_RS15810	3468966..3469517	+	552	WP_003139878.1	XRE family transcriptional regulator	-
WPAWT_RS15815	3469514..3469912	-	399	WP_023089053.1	NADH-quinone oxidoreductase subunit A	-
WPAWT_RS15820	3469925..3470248	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
WPAWT_RS15825	3470579..3471040	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
WPAWT_RS15830	3471045..3473240	+	2196	WP_043232957.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
WPAWT_RS15835	3473398..3473952	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
WPAWT_RS15840	3474041..3474619	+	579	WP_003110488.1	HD domain-containing protein	Toxin
WPAWT_RS15845	3474729..3475916	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
WPAWT_RS15850	3475906..3478077	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
WPAWT_RS15855	3478067..3479344	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T150658 WP_003110488.1 NZ_CP049161:3474041-3474619 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T150658 NZ_CP064868:955948-956319 [Mammaliicoccus sciuri]
ATGAGAAGAGGAGATGTATATCTCGCAGATCTTTCACCAGTTACAGGTTCTGAACAAGGGGGGACAAGACCAGTCGTTAT
TATCCAGAATGATACAGGTAATCGATACAGCCCGACTGTGATTGTTGCAGCAATTACTGGTAAGATAAATAAAGCCAAAA
TTCCCACTCATGTAGAAATTGAGGCAGCGAAATATAAATTAGACAGAGATTCCGTAATTTTGCTTGAACAAATCAGGACA
ATTGATAAAAAACGCTTAAAAGAAAAATTAACATATTTATCTGATGCAAAGATGAAAGAGGTTGATATCGCTATTGCCAT
CAGCCTCAATCTAACTTTGCAACAAAAATTTGATACATTAGGTAATACATAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT150658 WP_003088112.1 NZ_CP049161:3473398-3473952 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT150658 NZ_CP064868:955780-955947 [Mammaliicoccus sciuri]
ATGACATCTTTAACGCAAAATCGTACACAAAGTTTAGAACAAACACTGAAAGAAGGCTATCGTTCAATGGCCGATTTAAA
TCTCTCCCTTGCATCTGAAGCATACTATTGTGAATGTGAAGCTTGTGATTCAAATGAATCGCATATAGCCTCTCAGCAAG
GTGAATAA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4