Detailed information of TA system TA150650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 926479..927044
• Replicon: plasmid pSBC1_1
• Accession: NZ_CP049157
• Organism: Caballeronia sp. SBC1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SBC1_RS22230
• Protein ID: WP_165096149.1
• Coordinates: 926673..927044 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: SBC1_RS22225
• Protein ID: WP_165096154.1
• Coordinates: 926479..926676 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SBC1_RS22205	922089..923126	-	1038	WP_165096169.1	ABC transporter substrate-binding protein	-
SBC1_RS22210	923188..924414	-	1227	WP_165096166.1	Xaa-Pro peptidase family protein	-
SBC1_RS22215	924445..925332	-	888	WP_165096162.1	hypothetical protein	-
SBC1_RS22220	925450..926352	+	903	WP_165096159.1	LysR family transcriptional regulator	-
SBC1_RS22225	926479..926676	+	198	WP_165096154.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SBC1_RS22230	926673..927044	+	372	WP_165096149.1	PIN domain-containing protein	Toxin
SBC1_RS22235	927129..927674	+	546	WP_165096146.1	GNAT family N-acetyltransferase	-
SBC1_RS22240	927671..928603	-	933	WP_165096143.1	LysR family transcriptional regulator	-
SBC1_RS22245	928600..930156	-	1557	WP_165096139.1	cache domain-containing protein	-
SBC1_RS22250	930559..931329	+	771	WP_165096136.1	putative hydro-lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..1996666	1996666

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13126.03 Da
• Isoelectric Point: 6.0637

>T150650 WP_165096149.1 NZ_CP049157:926673-927044 [Caballeronia sp. SBC1]
MILVDTSIWIDHLRAGNAVLTDLLLSGRVLTHPFVIGELALGNLSQSDRVLESLLNLPRAVVATPEETVDFISSAALSGL
GIGYVDASLLASTRLTPDARLWTRDKRLHAAADRLGSSATVSH

Nucleotide

• Length: 372 bp

>T150650 NZ_CP064855:c3195609-3195451 [Erwinia amylovora]
ATGAAACTGCCAGCGAACTGTCTTATCTGGTGCGTACTTATAGTGTGTGTCACGCTTTTAATATTCACATATCTGACACG
AAAATCGCTCTGCGAAATTCGCTATAAGGATGGTTACAGGGAGGTGGCCGCTTTCATGGCTTACGAATCCGGCAAGTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7147.27 Da
• Isoelectric Point: 10.9435

>AT150650 WP_165096154.1 NZ_CP049157:926479-926676 [Caballeronia sp. SBC1]
MRTTIALDDDLLAKAQAYTGLQEKSALVREALKALIERESARRLARLGGSEPKLSPIPRRQSSKA

Nucleotide

• Length: 198 bp

>AT150650 NZ_CP064855:3195663-3195709 [Erwinia amylovora]
GCATGCAGATGGCCTCGTGGATTAATGAAAATTAACTACGGGGCTTT