Detailed information of TA system TA150644

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3527947..3528610
• Replicon: chromosome
• Accession: NZ_CP049154
• Organism: Cronobacter sakazakii strain C105731

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: G4U59_RS16555
• Protein ID: WP_032987888.1
• Coordinates: 3527947..3528363 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A3Q9CFW7
• Locus tag: G4U59_RS16560
• Protein ID: WP_007870701.1
• Coordinates: 3528344..3528610 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4U59_RS16535 (G4U59_16465)	3523935..3525668	-	1734	WP_007870697.1	single-stranded-DNA-specific exonuclease RecJ	-
G4U59_RS16540 (G4U59_16470)	3525672..3526391	-	720	WP_004385678.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
G4U59_RS16545 (G4U59_16475)	3526414..3527310	-	897	WP_087600150.1	site-specific tyrosine recombinase XerD	-
G4U59_RS16550 (G4U59_16480)	3527412..3527933	+	522	WP_004385676.1	flavodoxin FldB	-
G4U59_RS16555 (G4U59_16485)	3527947..3528363	-	417	WP_032987888.1	protein YgfX	Toxin
G4U59_RS16560 (G4U59_16490)	3528344..3528610	-	267	WP_007870701.1	FAD assembly factor SdhE	Antitoxin
G4U59_RS16565 (G4U59_16495)	3528888..3529877	+	990	WP_069681646.1	tRNA-modifying protein YgfZ	-
G4U59_RS16570 (G4U59_16500)	3529982..3530635	-	654	WP_007777939.1	hemolysin III family protein	-
G4U59_RS16575 (G4U59_16505)	3530778..3531092	-	315	WP_104658878.1	N(4)-acetylcytidine aminohydrolase	-
G4U59_RS16580 (G4U59_16510)	3531305..3532033	+	729	WP_085660912.1	MurR/RpiR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16441.44 Da
• Isoelectric Point: 11.4887

>T150644 WP_032987888.1 NZ_CP049154:c3528363-3527947 [Cronobacter sakazakii]
VVLWHSDLRVSWRSQWLSLLLHGAVAVIILLLPWPLRYLPVWMLLLSLVVFDCVRSQRRINAFQGEVSLTADYHLRWQGV
DWQICATPWMLRSGMMLRLRHPKTTRRHHVWLAADSMTEAEWRDLRRLLLQQPVGDKR

Nucleotide

• Length: 417 bp

>T150644 NZ_CP064855:472194-472469 [Erwinia amylovora]
ATGGAAATACAGTGGACCCAAAAGGCCCAGGATGACCTGGAGCGTATTTATAGGTTTGCCTGTCAGTACAGTCGGCAACA
TGCTGACAACGTATTAGAGCGTTTGCTCATAGGAACCTCAGGGCTCGCCGACCATCCAAGAATCGGTGTTTCTCAGATAA
GATATGAACCACGAGAAGTGAGAAAAATCTTGTTTGATGATTATGACGTTCACTATGAAATTCAGCGCGACACTATCTAT
ATTGTTGACCTTTGGCATACCCGAGAGGATCGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10411.89 Da
• Isoelectric Point: 4.8310

>AT150644 WP_007870701.1 NZ_CP049154:c3528610-3528344 [Cronobacter sakazakii]
MDINNKARIHWACRRGMRELDISIMPFFEYDYDSLSDEDKRVFVRLLECDDPDLFNWLMNHGKPADAELARMVELIQQRN
QARGPVAL

Nucleotide

• Length: 267 bp

>AT150644 NZ_CP064855:471942-472190 [Erwinia amylovora]
ATGAGACAACTCGCGAAACAGACGATCAGCGCTCAGATCCCTGCAGAGCTTGCCGCGGCGGTTGAAAACCTGGCTATTGA
ACTGGACAGATCTAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCCATGATTGAAGAGAGAGAGCGGCGGCATCAGCAGA
TTTTGAAAGGGCTTGCTGATGTCGATGCGGGAAGAGTGGTTAGCCATGCGGATGTAGTTGACTTCGCTAACAAACTGAAA
AAATCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3Q9CFW7