Detailed information of TA system TA150618

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2077768..2078431
• Replicon: chromosome
• Accession: NZ_CP049144
• Organism: Cronobacter sakazakii strain C79

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: G4U62_RS09800
• Protein ID: WP_069681645.1
• Coordinates: 2078015..2078431 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A3Q9CFW7
• Locus tag: G4U62_RS09795
• Protein ID: WP_007870701.1
• Coordinates: 2077768..2078034 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4U62_RS09770 (G4U62_09765)	2072797..2074230	-	1434	WP_148736390.1	6-phospho-beta-glucosidase	-
G4U62_RS09775 (G4U62_09770)	2074382..2075110	-	729	WP_069681659.1	MurR/RpiR family transcriptional regulator	-
G4U62_RS09780 (G4U62_09775)	2075286..2075600	+	315	WP_069681647.1	N(4)-acetylcytidine aminohydrolase	-
G4U62_RS09785 (G4U62_09780)	2075743..2076396	+	654	WP_007777939.1	hemolysin III family protein	-
G4U62_RS09790 (G4U62_09785)	2076501..2077490	-	990	WP_097582881.1	tRNA-modifying protein YgfZ	-
G4U62_RS09795 (G4U62_09790)	2077768..2078034	+	267	WP_007870701.1	FAD assembly factor SdhE	Antitoxin
G4U62_RS09800 (G4U62_09795)	2078015..2078431	+	417	WP_069681645.1	protein YgfX	Toxin
G4U62_RS09805 (G4U62_09800)	2078445..2078966	-	522	WP_004385676.1	flavodoxin FldB	-
G4U62_RS09810 (G4U62_09805)	2079068..2079964	+	897	WP_007892593.1	site-specific tyrosine recombinase XerD	-
G4U62_RS09815 (G4U62_09810)	2079987..2080706	+	720	WP_004385678.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
G4U62_RS09820 (G4U62_09815)	2080710..2082443	+	1734	WP_216310314.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16407.42 Da
• Isoelectric Point: 11.4887

>T150618 WP_069681645.1 NZ_CP049144:2078015-2078431 [Cronobacter sakazakii]
VVLWHSDLRVSWRSQWLSLLLHGAVAVIILLLPWPLRYLPVWMLLLSLVVFDCVRSQRRINALQGEVSLTADYHLRWQGV
DWQICATPWMLRSGMMLRLRHPKTTRRHHVWLAADSMTEAEWRDLRRLLLQQPVGDKR

Nucleotide

• Length: 417 bp

>T150618 NZ_CP064847:586872-587159 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10411.89 Da
• Isoelectric Point: 4.8310

>AT150618 WP_007870701.1 NZ_CP049144:2077768-2078034 [Cronobacter sakazakii]
MDINNKARIHWACRRGMRELDISIMPFFEYDYDSLSDEDKRVFVRLLECDDPDLFNWLMNHGKPADAELARMVELIQQRN
QARGPVAL

Nucleotide

• Length: 267 bp

>AT150618 NZ_CP064847:587146-587448 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCCTCGGTGCGTATTGATTCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAATTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A3Q9CFW7