Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 101378..101997 Replicon chromosome
Accession NZ_CP049144
Organism Cronobacter sakazakii strain C79

Toxin (Protein)


Gene name Hha Uniprot ID A0A2S9UIC9
Locus tag G4U62_RS00450 Protein ID WP_004386909.1
Coordinates 101779..101997 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3Q9CL77
Locus tag G4U62_RS00445 Protein ID WP_004386908.1
Coordinates 101378..101752 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4U62_RS00435 (G4U62_00430) 96539..97744 + 1206 WP_148737222.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
G4U62_RS00440 (G4U62_00435) 97766..100915 + 3150 WP_007848020.1 multidrug efflux RND transporter permease subunit AcrB -
G4U62_RS00445 (G4U62_00440) 101378..101752 + 375 WP_004386908.1 Hha toxicity modulator TomB Antitoxin
G4U62_RS00450 (G4U62_00445) 101779..101997 + 219 WP_004386909.1 HHA domain-containing protein Toxin
G4U62_RS00455 (G4U62_00450) 102203..102754 + 552 WP_148737220.1 maltose O-acetyltransferase -
G4U62_RS00460 (G4U62_00455) 102875..103384 + 510 WP_007891920.1 YlaC family protein -
G4U62_RS00465 (G4U62_00460) 103413..103553 - 141 WP_004386911.1 type B 50S ribosomal protein L36 -
G4U62_RS00470 (G4U62_00465) 103557..103817 - 261 WP_004386912.1 type B 50S ribosomal protein L31 -
G4U62_RS00475 (G4U62_00470) 103957..105858 - 1902 WP_105622652.1 methyl-accepting chemotaxis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8598.02 Da        Isoelectric Point: 9.4828

>T150615 WP_004386909.1 NZ_CP049144:101779-101997 [Cronobacter sakazakii]
MTTKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Download         Length: 219 bp

>T150615 NZ_CP064847:c314176-313799 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14343.05 Da        Isoelectric Point: 4.7168

>AT150615 WP_004386908.1 NZ_CP049144:101378-101752 [Cronobacter sakazakii]
MDEYSPKRHDIAQLKFLCESLYHDCLATLGENQHGWVNDPTSAVNLQLNDLIEHIASFGLNYKIKHEDDAALIEQLDEYL
DDTFMLFSNYGINAQDLQKWRKSGNRLFRCFVNASKENPVSLSF

Download         Length: 375 bp

>AT150615 NZ_CP064847:c314634-314266 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S9UIC9

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q9CL77

References