Detailed information of TA system TA150609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1362783..1363470
• Replicon: chromosome
• Accession: NZ_CP049140
• Organism: Pseudomonas nitroreducens strain HBP1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: G5B91_RS06445
• Protein ID: WP_038803249.1
• Coordinates: 1362783..1363196 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6G6ISI0
• Locus tag: G5B91_RS06450
• Protein ID: WP_037008289.1
• Coordinates: 1363222..1363470 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5B91_RS06440	1360703..1362745	+	2043	WP_024767531.1	exodeoxyribonuclease V subunit alpha	-
G5B91_RS06445	1362783..1363196	-	414	WP_038803249.1	type II toxin-antitoxin system VapC family toxin	Toxin
G5B91_RS06450	1363222..1363470	-	249	WP_037008289.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
G5B91_RS06455	1363628..1366474	+	2847	WP_024767534.1	response regulator	-
G5B91_RS06460	1366453..1367649	+	1197	WP_024767535.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14872.07 Da
• Isoelectric Point: 5.1219

>T150609 WP_038803249.1 NZ_CP049140:c1363196-1362783 [Pseudomonas nitroreducens]
MLDTNICSFIMRQNPPQVLQRLQKAAEAGNRLVISAITHAELRYGAASPKAPKAVAAWIDALVQRLDDVLPWDIDAVDSS
AKLMRLLLDSGTPIGPNDTGIAGHVLATDCVIVTNNTREFRRVPGLVVEDWCNSEPA

Nucleotide

• Length: 414 bp

>T150609 NZ_CP064832:939613-939840 [Morganella morganii subsp. morganii]
ATGGCAAGGACGATTGAACGTTCATCTCAGTTTAAAAAAGATTATAAACGGGAAGTTAAGGGTATTTACCGTATGATGTT
AAATGACAGTCTACAGAACATCCTGAATATATTGGTAAATGATCTCCCTATACCTGAAAAATACGCTGATCATCCGCTTA
AAGGAAATTGGCGTACATTTCGTGATTGCCACCTCTATCCTGATTTGATTTTGATTTATAAAAAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8946.06 Da
• Isoelectric Point: 4.4091

>AT150609 WP_037008289.1 NZ_CP049140:c1363470-1363222 [Pseudomonas nitroreducens]
MPTTTVSLFRNGKNQAVRIPRDMEFKGVTELVMTREGDTLIQRPSRPTGSSLAELEPVDADFLGDRPDVIAPGRLGFGDD
EG

Nucleotide

• Length: 249 bp

>AT150609 NZ_CP064832:939357-939629 [Morganella morganii subsp. morganii]
GTGGAATATGTCAGAGCAAAAATTGATCCGAAGCTGAAAGCAGAAGCGATTGAAACGCTTGAATCGATGGGCTTAACAGT
GAGTGACGCGATAAGAATGTTGATGATTCAGATTGTTGAACAGAAAGCGATGCCATTTTGTGTGAAAAGTACACAATATC
GTCATGCTGCACGTACCCGGGCCGCAATAGAAGAGTTGGAGTGCGGGAAAGGCAAAAAATTTGACAGTATTTCAGATTTA
ATGGCGGATCTGCAGGATGGCAAGGACGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G6ISI0