Detailed information of TA system TA150603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 69161..69738
• Replicon: chromosome
• Accession: NZ_CP049139
• Organism: Herbaspirillum frisingense strain IAC152

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G5S34_RS00290
• Protein ID: WP_006465342.1
• Coordinates: 69161..69526 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7G6AQT8
• Locus tag: G5S34_RS00295
• Protein ID: WP_006465343.1
• Coordinates: 69520..69738 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S34_RS00265	64853..65104	+	252	WP_102664360.1	ferrous iron transport protein A	-
G5S34_RS00270	65101..66927	+	1827	WP_182993454.1	ferrous iron transporter B	-
G5S34_RS00275	66924..67208	+	285	WP_182993455.1	hypothetical protein	-
G5S34_RS00280	67236..68441	-	1206	WP_006465340.1	threonine ammonia-lyase	-
G5S34_RS00285	68789..69139	+	351	WP_182993456.1	hypothetical protein	-
G5S34_RS00290	69161..69526	-	366	WP_006465342.1	type II toxin-antitoxin system VapC family toxin	Toxin
G5S34_RS00295	69520..69738	-	219	WP_006465343.1	ribbon-helix-helix protein, CopG family	Antitoxin
G5S34_RS00300	69880..70452	+	573	WP_006465344.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
G5S34_RS00305	70485..71350	-	866	Protein_60	ABC transporter permease	-
G5S34_RS00310	71359..72222	-	864	WP_182993457.1	ABC transporter ATP-binding protein	-
G5S34_RS00315	72203..73138	-	936	WP_202032483.1	ABC transporter substrate-binding protein	-
G5S34_RS00320	73230..74228	-	999	WP_182993459.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13863.06 Da
• Isoelectric Point: 8.4752

>T150603 WP_006465342.1 NZ_CP049139:c69526-69161 [Herbaspirillum frisingense]
MVKALFDTNILVDYLNGIVAAKKEMARYEYRAISIITWMEVMVGASEDNEEALRSWLSSFHLINLDSTVAQRAVMIRQQR
RIRLPDAIVWASAQEHSLLLVSRNTKDFPANEPGVRVPYKL

Nucleotide

• Length: 366 bp

>T150603 NZ_CP064829:3574267-3574590 [Morganella morganii subsp. morganii]
ATGGATTACCTGGAATTTATCGAAACTAAAATTTTCAGCCATACCCGCAAAGAAATGATGACTGATGATGAATTCCGGGA
ATTTCAGAGCTATCTTTTGGAAAACTACGAACGGGGTAACACGATCAGTCAGACAGGCGGATGTAAGAAGATTCGCTGGC
AACGCAGGGGAACCGGTAAACAAGGCGGCCTCCGGGTCATTTATCACGCCCTTCCCGCTCACGGCAGATTGTACCTGCTT
CTTATCTACCCCAAAAATGTCAAAGATGATCTGAGTAGTCAGGAAAGAACGGTGCTCAGATCGTTATCACAGAAACTGAA
CTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8314.56 Da
• Isoelectric Point: 7.2019

>AT150603 WP_006465343.1 NZ_CP049139:c69738-69520 [Herbaspirillum frisingense]
MSRILVDIPDSQLAELTQIVDSQQRPRAVVIREAIAAYIAQHKPEHDAEVFGLWKKKKVDGLAYQKELRSEW

Nucleotide

• Length: 219 bp

>AT150603 NZ_CP064829:3574636-3574941 [Morganella morganii subsp. morganii]
ATGGATAAAACACTTTTTGAAAATCTGGTCAGCAGCATGGAAGAGATGGTGGCAATTGAAAAAGGCCACGTTGTTCCGGC
ACAGGAAAACAGGCACAGACACCCGATTCCGGATGTTAAAGCAATCCGCAAGCATGCCGGATTAAAACAGGCAGAATTTG
CAGATGCTATCGGTTCCAGTGTTGATCTGGTCCGCAGTTGGGAGCAGCATCGTCGTATTCCGTCAGGGATTGCCTTAAAA
ATGTTGTATCTCATTGAAAAACAACCCGCACTTTTATGTACACTACGATCAATCCCTCTGCGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7G6AQT8