Detailed information of TA system TA150589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 104808..105433
• Replicon: plasmid pEC931_1
• Accession: NZ_CP049119
• Organism: Escherichia coli strain EC931

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6G93_RS23695
• Protein ID: WP_000911313.1
• Coordinates: 105035..105433 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: G6G93_RS23690
• Protein ID: WP_000450520.1
• Coordinates: 104808..105035 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6G93_RS23690	104808..105035	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
G6G93_RS23695	105035..105433	+	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
G6G93_RS23700	105442..107595	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
G6G93_RS23705	107848..108579	-	732	WP_023565183.1	complement resistance protein TraT	-
G6G93_RS23710	108611..109108	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(B) / catA1 / sul2 / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..159690	159690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T150589 WP_000911313.1 NZ_CP049119:105035-105433 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T150589 NZ_CP064825:4625019-4625192 [Escherichia coli UTI89]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT150589 WP_000450520.1 NZ_CP049119:104808-105035 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT150589 NZ_CP064825:4624695-4624991 [Escherichia coli UTI89]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: V0VCB2