Detailed information of TA system TA150584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 41058..41434
• Replicon: plasmid pEC931_1
• Accession: NZ_CP049119
• Organism: Escherichia coli strain EC931

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: G6G93_RS23345
• Protein ID: WP_000969988.1
• Coordinates: 41058..41240 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: G6G93_RS23350
• Protein ID: WP_001324224.1
• Coordinates: 41237..41434 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6G93_RS23330	37767..38464	+	698	Protein_28	IS1 family transposase	-
G6G93_RS23335	39058..40092	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
G6G93_RS23340	40651..40959	-	309	WP_000377483.1	hypothetical protein	-
G6G93_RS23345	41058..41240	-	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G6G93_RS23350	41237..41434	-	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
G6G93_RS23355	42116..43390	+	1275	WP_000489609.1	HlyD family secretion protein	-
G6G93_RS23360	43365..45479	+	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
G6G93_RS23365	45649..45960	-	312	WP_001259758.1	colicin V	-
G6G93_RS23370	45938..46174	-	237	WP_001323890.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(B) / catA1 / sul2 / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..159690	159690
	-	flank	IS/Tn	-	-	37961..38464	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T150584 WP_000969988.1 NZ_CP049119:c41240-41058 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T150584 NZ_CP064825:c3219489-3219082 [Escherichia coli UTI89]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT150584 WP_001324224.1 NZ_CP049119:c41434-41237 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT150584 NZ_CP064825:c3219736-3219470 [Escherichia coli UTI89]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3