Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3937350..3937967 Replicon chromosome
Accession NZ_CP049115
Organism Pantoea stewartii strain ZJ-FGZX1

Toxin (Protein)


Gene name Hha Uniprot ID H3R994
Locus tag G5574_RS18520 Protein ID WP_006117940.1
Coordinates 3937350..3937565 (-) Length 72 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A6G6JU23
Locus tag G5574_RS18525 Protein ID WP_039338473.1
Coordinates 3937590..3937967 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5574_RS18490 3933249..3933587 + 339 WP_003850469.1 P-II family nitrogen regulator -
G5574_RS18495 3933621..3934907 + 1287 WP_033738675.1 ammonium transporter AmtB -
G5574_RS18500 3934956..3935819 - 864 WP_006117944.1 acyl-CoA thioesterase II -
G5574_RS18505 3936012..3936569 + 558 WP_006117943.1 YbaY family lipoprotein -
G5574_RS18510 3936610..3936951 - 342 WP_152998336.1 MGMT family protein -
G5574_RS18520 3937350..3937565 - 216 WP_006117940.1 hemolysin expression modulator Hha Toxin
G5574_RS18525 3937590..3937967 - 378 WP_039338473.1 Hha toxicity modulator TomB Antitoxin
G5574_RS18530 3938114..3938467 - 354 WP_033738682.1 hypothetical protein -
G5574_RS18535 3938817..3939485 + 669 WP_033738793.1 ATP-binding cassette domain-containing protein -
G5574_RS18540 3939482..3940321 + 840 WP_006117933.1 metal ABC transporter permease -
G5574_RS18545 3940334..3941212 + 879 WP_033738684.1 metal ABC transporter substrate-binding protein -
G5574_RS18550 3941270..3941410 - 141 WP_006117931.1 type B 50S ribosomal protein L36 -
G5574_RS18555 3941422..3941676 - 255 WP_164483024.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(12-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8528.91 Da        Isoelectric Point: 9.4825

>T150582 WP_006117940.1 NZ_CP049115:c3937565-3937350 [Pantoea stewartii]
MNKTLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVSVWKFVR

Download         Length: 216 bp

>T150582 NZ_CP064825:c2228120-2227869 [Escherichia coli UTI89]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14622.23 Da        Isoelectric Point: 4.4699

>AT150582 WP_039338473.1 NZ_CP049115:c3937967-3937590 [Pantoea stewartii]
MDEYSPKRHDIAQLKYVCESLFDASMATLTDSHHGWVNDPTADGNLQLNDLIEHIASFTMNYKIKHAEDEALITQIDEYL
DDTFMLFSNYSVNTQDLQRWQRSAKRLFNLFQEECAYLQQPSHSL

Download         Length: 378 bp

>AT150582 NZ_CP064825:c2227872-2227618 [Escherichia coli UTI89]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H3R994

Antitoxin

Source ID Structure
AlphaFold DB A0A6G6JU23

References