Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 20438..22061 Replicon plasmid pWF5_MCR
Accession NZ_CP049114
Organism Escherichia coli strain WF5-5-T2

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag G6R06_RS23565 Protein ID WP_000088046.1
Coordinates 20438..21763 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag G6R06_RS23570 Protein ID WP_024136327.1
Coordinates 21768..22061 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6R06_RS23535 (G6R06_23375) 16422..17279 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
G6R06_RS23540 (G6R06_23380) 17295..17603 - 309 WP_001224687.1 hypothetical protein -
G6R06_RS23545 (G6R06_23385) 18152..18577 - 426 WP_001043843.1 hypothetical protein -
G6R06_RS23550 (G6R06_23390) 18831..19646 - 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
G6R06_RS23555 (G6R06_23395) 19659..20069 - 411 WP_000985911.1 hypothetical protein -
G6R06_RS23560 (G6R06_23400) 20171..20377 - 207 WP_000134171.1 hypothetical protein -
G6R06_RS23565 (G6R06_23405) 20438..21763 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
G6R06_RS23570 (G6R06_23410) 21768..22061 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
G6R06_RS23575 (G6R06_23415) 22665..23057 - 393 WP_000341066.1 hypothetical protein -
G6R06_RS23580 (G6R06_23420) 23430..23771 + 342 WP_001151575.1 hypothetical protein -
G6R06_RS23585 (G6R06_23425) 23863..24054 + 192 WP_000398480.1 hypothetical protein -
G6R06_RS23590 (G6R06_23430) 24104..24385 + 282 WP_000077926.1 hypothetical protein -
G6R06_RS23595 24726..24857 - 132 WP_000374059.1 hypothetical protein -
G6R06_RS23600 (G6R06_23435) 24948..26189 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid fosA3 / blaCTX-M-14 / mcr-1.1 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / sul2 / aph(4)-Ia / aac(3)-IVa / mph(A) / tet(M) - 1..254290 254290


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T150581 WP_000088046.1 NZ_CP049114:c21763-20438 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T150581 NZ_CP064825:c2227872-2227618 [Escherichia coli UTI89]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT150581 WP_024136327.1 NZ_CP049114:c22061-21768 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT150581 NZ_CP064825:c2228120-2227869 [Escherichia coli UTI89]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References