Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4106917..4107414 Replicon chromosome
Accession NZ_CP049112
Organism Escherichia coli strain WF5-5-T2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G6R06_RS20140 Protein ID WP_001173343.1
Coordinates 4106917..4107090 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag G6R06_RS20145 Protein ID WP_000398619.1
Coordinates 4107118..4107414 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6R06_RS20130 (4103587) 4103587..4105044 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
G6R06_RS20135 (4105281) 4105281..4106798 + 1518 WP_001295074.1 lysine--tRNA ligase -
G6R06_RS20140 (4106917) 4106917..4107090 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G6R06_RS20145 (4107118) 4107118..4107414 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G6R06_RS20150 (4107641) 4107641..4107913 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
G6R06_RS20155 (4107925) 4107925..4108155 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
G6R06_RS20160 (4108336) 4108336..4109967 + 1632 WP_001216477.1 sensor histidine kinase -
G6R06_RS20165 (4109964) 4109964..4110683 + 720 Protein_3937 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T150579 WP_001173343.1 NZ_CP049112:c4107090-4106917 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T150579 NZ_CP064825:c1949896-1949794 [Escherichia coli UTI89]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT150579 WP_000398619.1 NZ_CP049112:c4107414-4107118 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT150579 NZ_CP064825:1949756-1950024 [Escherichia coli UTI89]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References