Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 3498525..3499204 | Replicon | chromosome |
| Accession | NZ_CP049112 | ||
| Organism | Escherichia coli strain WF5-5-T2 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | G6R06_RS17105 | Protein ID | WP_000854672.1 |
| Coordinates | 3498525..3498866 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | G6R06_RS17110 | Protein ID | WP_000070395.1 |
| Coordinates | 3498887..3499204 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G6R06_RS17080 (3494111) | 3494111..3494203 | + | 93 | Protein_3336 | sigma factor-binding protein Crl | - |
| G6R06_RS17085 (3494242) | 3494242..3495297 | - | 1056 | WP_103119219.1 | phosphoporin PhoE | - |
| G6R06_RS17090 (3495585) | 3495585..3496688 | + | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| G6R06_RS17095 (3496700) | 3496700..3497953 | + | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| G6R06_RS17105 (3498525) | 3498525..3498866 | - | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| G6R06_RS17110 (3498887) | 3498887..3499204 | - | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| G6R06_RS17115 (3499223) | 3499223..3499444 | - | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| G6R06_RS17120 (3499453) | 3499453..3499929 | - | 477 | WP_000811693.1 | RadC family protein | - |
| G6R06_RS17125 (3499945) | 3499945..3500403 | - | 459 | WP_000211838.1 | antirestriction protein | - |
| G6R06_RS17130 (3500501) | 3500501..3500740 | - | 240 | WP_000194654.1 | DUF905 family protein | - |
| G6R06_RS17135 (3500817) | 3500817..3501284 | - | 468 | WP_001547765.1 | protein YkfB | - |
| G6R06_RS17140 (3501307) | 3501307..3501750 | - | 444 | WP_000824223.1 | lipoprotein YafY | - |
| G6R06_RS17145 (3501750) | 3501750..3501977 | - | 228 | WP_001548158.1 | protein YpjK | - |
| G6R06_RS17150 (3501973) | 3501973..3502164 | - | 192 | Protein_3349 | DeoR family transcriptional regulator | - |
| G6R06_RS17155 (3502381) | 3502381..3503202 | - | 822 | WP_000197389.1 | DUF932 domain-containing protein | - |
| G6R06_RS17160 (3503294) | 3503294..3504157 | - | 864 | WP_001065553.1 | GTPase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 3498305..3517168 | 18863 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T150572 WP_000854672.1 NZ_CP049112:c3498866-3498525 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T150572 NZ_CP064825:284300-284698 [Escherichia coli UTI89]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT150572 WP_000070395.1 NZ_CP049112:c3499204-3498887 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT150572 NZ_CP064825:284004-284297 [Escherichia coli UTI89]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|