Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2375034..2375672 Replicon chromosome
Accession NZ_CP049112
Organism Escherichia coli strain WF5-5-T2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag G6R06_RS11575 Protein ID WP_000813794.1
Coordinates 2375496..2375672 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag G6R06_RS11570 Protein ID WP_001270286.1
Coordinates 2375034..2375450 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6R06_RS11550 (2370186) 2370186..2371127 - 942 WP_001251304.1 ABC transporter permease -
G6R06_RS11555 (2371128) 2371128..2372141 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
G6R06_RS11560 (2372159) 2372159..2373304 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
G6R06_RS11565 (2373549) 2373549..2374955 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
G6R06_RS11570 (2375034) 2375034..2375450 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
G6R06_RS11575 (2375496) 2375496..2375672 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
G6R06_RS11580 (2375894) 2375894..2376124 + 231 WP_000494244.1 YncJ family protein -
G6R06_RS11585 (2376216) 2376216..2378177 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
G6R06_RS11590 (2378250) 2378250..2378786 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
G6R06_RS11595 (2378878) 2378878..2380053 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T150570 WP_000813794.1 NZ_CP049112:c2375672-2375496 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T150570 NZ_CP064825:c15663-15454 [Escherichia coli UTI89]
ATGCAGAACACATGTAGAGTGCCTCTTACTGACCGTAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATGAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT150570 WP_001270286.1 NZ_CP049112:c2375450-2375034 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT150570 NZ_CP064825:15655-15712 [Escherichia coli UTI89]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References