Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1805815..1806646 Replicon chromosome
Accession NZ_CP049112
Organism Escherichia coli strain WF5-5-T2

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag G6R06_RS08665 Protein ID WP_000854814.1
Coordinates 1805815..1806189 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag G6R06_RS08670 Protein ID WP_001285584.1
Coordinates 1806278..1806646 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6R06_RS08625 (1801211) 1801211..1802377 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
G6R06_RS08630 (1802496) 1802496..1802969 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
G6R06_RS08635 (1803167) 1803167..1804225 + 1059 WP_001200891.1 FUSC family protein -
G6R06_RS08640 (1804397) 1804397..1804726 + 330 WP_000450409.1 DUF496 family protein -
G6R06_RS08645 (1804827) 1804827..1804961 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
G6R06_RS08650 (1805081) 1805081..1805209 + 129 Protein_1689 transposase domain-containing protein -
G6R06_RS08655 (1805498) 1805498..1805578 - 81 Protein_1690 hypothetical protein -
G6R06_RS08660 (1805624) 1805624..1805818 - 195 WP_000988600.1 DUF5983 family protein -
G6R06_RS08665 (1805815) 1805815..1806189 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
G6R06_RS08670 (1806278) 1806278..1806646 - 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
G6R06_RS08675 (1806720) 1806720..1806941 - 222 WP_000692323.1 DUF987 domain-containing protein -
G6R06_RS08680 (1807004) 1807004..1807450 - 447 WP_000187523.1 RadC family protein -
G6R06_RS08685 (1807447) 1807447..1808979 - 1533 WP_001350525.1 protein YeeR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T150559 WP_000854814.1 NZ_CP049112:c1806189-1805815 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T150559 NZ_CP064794:4541972-4542124 [Xanthomonas oryzae pv. oryzicola]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT150559 WP_001285584.1 NZ_CP049112:c1806646-1806278 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT150559 NZ_CP064794:4542199-4542333 [Xanthomonas oryzae pv. oryzicola]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References